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Plasmid#13540DepositorInsertFLIP-E A207W
UseTagsECFP and VenusExpressionBacterialMutationA207W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
His6_BcLOV4_Q355N_mCherry_BamUK
Plasmid#119762PurposeEncodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cellDepositorInsertBcLOV4-Q355N_mCherry
UseTagsmCherryExpressionBacterialMutationMutated glutamine at position 355 to asparaginePromoterT7Available sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDest472 SHP-1 S591D
Plasmid#21806DepositorInsertSHP-1 (PTPN6 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 591 to Aspartic AcidPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pMT-DREDD-V5-His
Plasmid#63749PurposeExpresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertDeath related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
UseTagsV5-6XHisExpressionInsectMutationPromoterpMTAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-100u
Plasmid#13539DepositorInsertFLIP-E S118W
UseTagsECFP and VenusExpressionBacterialMutationS118W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
piHGpd/G170D
Plasmid#1113DepositorInsertHSP82 (HSP82 Budding Yeast)
UseTagsExpressionYeastMutationHSP82 with glycine 170 changed to aspartic acid; …PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9-GFP S122D
Plasmid#114667PurposeExpresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationchanged serine 122 to aspartic acidPromoterATG9Available sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-fishRab8A[T22N]
Plasmid#24906DepositorInsertfishRab8A
UseGateway entry vectorTagsExpressionMutationChanged Threonine 22 to AsparaginePromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-fishRab8B[T22N]
Plasmid#24909DepositorInsertfishRab8B
UseGateway entry vectorTagsExpressionMutationChanged Threonine 22 to AsparaginePromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BubR1-D911A
Plasmid#200815Purposeexpresses BubR1-D911A in mammalian cellsDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
UseTagsFLAGExpressionMammalianMutationChange Aspartic acid 911 to AlaninePromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hTspan12LEL(118-218)_pAcGP67a
Plasmid#216383PurposeBaculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBPDepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3CExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549H 3xFlag
Plasmid#110106Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Asparagine 549 to Histidine (N549H)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549K 3xFlag
Plasmid#110107Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Asparagine 549 to Lysine (N549K)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK340
Plasmid#71837PurposeCloned region contains Acetobacter aceti 1023 purE(H59N) geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only