We narrowed to 1,197 results for: Aspa

1. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-NES-DEVD-mCardinal-NLS

   Plasmid

   #164052

   Purpose

   Fluorescent reporter for caspase-3

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-mCardinal-NLS
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET FLIPE-600n

   Plasmid

   #13537

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-Rab8A[T22N]

   Plasmid

   #24899

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A[T22N]
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 22 to Asparagine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. Casp1

   Plasmid

   #98849

   Purpose

   Expresses murine Casp1 in mammalian cells

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Casp1 (Casp1 Mouse)
   

   Use

   Tags

   No fusion proteins or tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECFP-DEVG-Venus

   Plasmid

   #24538

   Depositor

   Peter Sorger

   Article

   Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

   Insert

   ECFP-DEVG-Venus
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   DEVDR mutated to DEVG

   Promoter

   CMV

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pDisplay FLIPE-600n

   Plasmid

   #13545

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET23-CBD-(-PP)-EGFP

   Plasmid

   #12597

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   Cdc42 Biosensor (WAS Human)
   

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   F271C

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP-Rac2(N17)

    Plasmid

    #11398

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 changed to Asparagine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

    Plasmid

    #216382

    Purpose

    Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Tetraspanin-12 (TSPAN12 Human)
    

    Use

    Baculovirus

    Tags

    6xHis-3C-splitGFP11

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-EsaR-D91G

    Plasmid

    #47646

    Purpose

    pAC-EsaR with a a272g mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pEF1a-mRor2N620K

    Plasmid

    #22631

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

    Insert

    Ror2 N620K (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Asparagine 620 to Lysine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1 FLIPE-600n

    Plasmid

    #13541

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET FLIPE-10u

    Plasmid

    #13538

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSET FLIPE-1m

    Plasmid

    #13540

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His6_BcLOV4_Q355N_mCherry_BamUK

    Plasmid

    #119762

    Purpose

    Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

    Depositor

    Brian Chow

    Article

    Glantz et al Methods Enzymol. 2019;622:249-270. doi:

    Insert

    BcLOV4-Q355N_mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Mutated glutamine at position 355 to asparagine

    Promoter

    T7

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDest472 SHP-1 S591D

    Plasmid

    #21806

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Aspartic Acid

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

20. ASPH

    Plasmid

    #39094

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ASPH (ASPH Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only