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pL0_CD-mRFP1

Plasmid

#202145

Purpose

Level 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRFP1 gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-tagRFP

Plasmid

#203482

Purpose

For Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

TagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-pnRFP

Plasmid

#201678

Purpose

Bacteria protein expression of pnRFP biosensor

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-pnRFP

Plasmid

#201679

Purpose

Expresses pnRFP in mammalian cells

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP670-2-N1

Plasmid

#197233

Purpose

Expresses the fusion protein of Keratin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCx43-7-miRFP670-2-N1

Plasmid

#197223

Purpose

Expresses the fusion protein of Cx43-7-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP720-Actin-C-18

Plasmid

#197221

Purpose

Expresses the fusion protein of miRFP720-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCx43-7-miRFP680-N1

Plasmid

#197224

Purpose

Expresses the fusion protein of Cx43-7-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP713-Actin-C-18

Plasmid

#197220

Purpose

Expresses the fusion protein of miRFP713-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP720-N1

Plasmid

#197226

Purpose

Expresses the fusion protein of Cx43-7-miRFP720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP720

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-iRFP670-N1

Plasmid

#197222

Purpose

Expresses the fusion protein of Cx43-7-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP713-N1

Plasmid

#197225

Purpose

Expresses the fusion protein of Cx43-7-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-iRFP670-N1

Plasmid

#197227

Purpose

Expresses the fusion protein of H2B-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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piRFP670-Actin-C-18

Plasmid

#197217

Purpose

Expresses the fusion protein of iRFP670-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP670-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-2-Actin-C-18

Plasmid

#197218

Purpose

Expresses the fusion protein of miRFP670-2-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-miRFP680-N1

Plasmid

#197234

Purpose

Expresses the fusion protein of Keratin-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-iRFP670-N1

Plasmid

#197232

Purpose

Expresses the fusion protein of Keratin-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2miRFP670 pc3942

Plasmid

#167568

Purpose

The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

MeCp2 (Mecp2 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENmRFPCNAL2 pc1054

Plasmid

#167567

Purpose

The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

Depositor

Cristina Cardoso

Article

Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge