We narrowed to 1,212 results for: ncl

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pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pPEE17

Plasmid

#128361

Purpose

Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Insert

Acetamidase (amdS)

Use

Unspecified

Promoter

TEF1

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDH3

Plasmid

#83789

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Mutation

F64L, Y66W, N146I, M153T, V163A, N164H mutations …

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-SOS

Plasmid

#208279

Purpose

Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9GFP-shP53

Plasmid

#102906

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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CD44I-c001

Plasmid

#175448

Purpose

Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.

Depositor

Opher Gileadi

Insert

CD44 (CD44 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLY134

Plasmid

#130962

Purpose

Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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TR-5'UTR-HP-GFP

Plasmid

#80594

Purpose

Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Mutation

Inserted 30 nt hairpin which is targeted by Csy4 …

Promoter

CBA

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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psecBFP2

Plasmid

#62230

Purpose

encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

Depositor

Erik Snapp

Article

Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

Insert

secBFP2

Tags

secbfp2

Expression

Mammalian

Promoter

cmv

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
UAS::PTPRDb-DN-GFP

Plasmid

#37112

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)

Use

Tol2 gateway destination vector

Tags

EGFP

Mutation

deleted cytodomain, including phosphatase domains…

Promoter

UAS

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pJLI-SUP35 (delta)2-5

Plasmid

#1138

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted, but …

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLSLZ

Plasmid

#51495

Purpose

pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant t-dna plasmid

Tags

SV40 NLS

Promoter

virion-sense LIR and 2x35S

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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PHMcc(delta16 PHM 356s)

Plasmid

#25455

Depositor

Betty Eipper

Article

Kolhekar et al Biochemistry. 1997 Sep 9. 36(36):10901-9

Insert

PHM (Pam Rat)

Expression

Mammalian

Mutation

Contains the catalytic core of PHM, including aa …

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
p-mCherry

Plasmid

#249067

Purpose

Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-bleo-atpD

Plasmid

#248066

Purpose

Lentiviral expression of beta subunit of ATPGobble. Includes ampR and bleoR (zeocin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for active ATPGobble.

Depositor

Denis Titov

Article

Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.

Insert

atpD

Use

Lentiviral

Tags

FLAG

Mutation

codon-optimized for expression in human cells

Promoter

TRE3GS

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-NHis6NocM-emptyMCS2

Plasmid

#240399

Purpose

expresses N-His tagged NocM (acyl carrier protein) from Nodularia sp. 06071

Depositor

Emily Balskus

Article

Wang et al Biochemistry. 2025 May 20;64(10):2173-21

Insert

nocM

Tags

His

Expression

Bacterial

Mutation

M1 in native sequence not included in insert

Promoter

T7

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet-solo-NHis6-NocO

Plasmid

#240400

Purpose

expresses N-His tagged NocO (diiron halogenase) from Nodularia sp. 06071

Depositor

Emily Balskus

Article

Wang et al Biochemistry. 2025 May 20;64(10):2173-21

Insert

nocO

Tags

His

Expression

Bacterial

Mutation

M1 of native sequence not included in insert

Promoter

T7

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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