We narrowed to 17,199 results for: puro

1. pPBpuro-miRFP670-HRasCT

   Plasmid

   #176030

   Purpose

   Encoding membrane-targeted miRFP670

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   Membrane-targeted miRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti puro HA-LMNB1

   Plasmid

   #227591

   Purpose

   Lentiviral plasmid expressing human LMNB1 protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   LMNB1 (LMNB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVXPuro-mGFP-Sec23B

   Plasmid

   #66598

   Purpose

   Expresses Sec23B as a fusion with mGFP

   Depositor

   David Stephens

   Article

   Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

   Insert

   Sec23B (SEC23B Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 puro shYAP1-1

   Plasmid

   #193692

   Purpose

   Constitutive lentiviral expression of YAP1 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-GemininΔDbox-HA-Puro

   Plasmid

   #193770

   Purpose

   Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   GemininΔDbox-HA (GMNN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Dbox removed (R23A, L26A). Codons switched at siR…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. attP-puro-GA donor

   Plasmid

   #182140

   Purpose

   attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-puro-mycRSK2wt

   Plasmid

   #15827

   Depositor

   Jing Chen

   Article

   Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

   Insert

   Ribosomal S6 kinase 2 (Rps6ka3 Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro

    Plasmid

    #192001

    Purpose

    Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-Isoform1 (LYSET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVTRE3G

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-CTSB-puro

    Plasmid

    #158463

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-CMV-Puro-2A

    Plasmid

    #102611

    Purpose

    Control lentivector expressing Puro resistance and gene of interest from CMV promoter

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-Puro-CLDN18

    Plasmid

    #69466

    Purpose

    Expresses CLDN18

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    CLDN18 (CLDN18 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ609-dCas9-KR-puro

    Plasmid

    #158478

    Purpose

    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

    Depositor

    Brendan Price

    Article

    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

    Insert

    dCas9-KR
    

    Use

    CRISPR

    Tags

    KillerRed

    Expression

    Mammalian

    Mutation

    Cas9m4 (D10A+D839A+H840A+N863A)

    Promoter

    CMV

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLD-puro-Cn-VA-NHEJ1-WT

    Plasmid

    #141339

    Purpose

    Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    NHEJ1 (NHEJ1 Human)
    

    Use

    Lentiviral

    Tags

    VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH231-EF1-Puro

    Plasmid

    #115143

    Purpose

    Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Puromycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Tight-Puro_Nkx2.2

    Plasmid

    #64840

    Purpose

    A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx2.2 (Nkx2-2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCAR_sgRNA_puro-mKate-lox5171

    Plasmid

    #162077

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE puro Tusc5

    Plasmid

    #87952

    Purpose

    retroviral plasmid containing mouse Tusc5

    Depositor

    David James

    Article

    Fazakerley et al J Biol Chem. 2015 Sep 25;290(39):23528-4

    Insert

    Tumor Suppressor Candidate 5 (Tusc5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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