We narrowed to 1,082 results for: rbis

1. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

   Plasmid

   #172646

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Leu

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

   Plasmid

   #172633

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-2xFLAG-2xSTREP-CCND1-Puro

   Plasmid

   #172634

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry

   Plasmid

   #172636

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Leu

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro

   Plasmid

   #172617

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro284Leu

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro

   Plasmid

   #172624

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro281Leu

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR1A_mmFbxw7_R505C (closed)

   Plasmid

   #160103

   Purpose

   Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Fbxw7 alpha R505C (Fbxw7 Mouse)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   R505C

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTet1 flox donor

   Plasmid

   #117147

   Purpose

   Making Tet1 conditional knock out

   Depositor

   Jacob Hanna

   Article

   Zviran et al ssrn.3155731

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid

    #223113

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid

    #223114

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid

    #223115

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid

    #223116

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS SARS-CoV-2 BA.2.12.1 Spike

    Plasmid

    #186032

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike

    Depositor

    Marceline Côté

    Article

    Gong et al Viruses. 2022 Sep 30;14(10):2178. doi: 1

    Insert

    SARS-CoV-2 BA.2.12.1 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the BA.2 mutations (T19I, LPPA24S, G142D…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid

    #223079

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-human OCT4 PE-SV40-Luc

    Plasmid

    #52415

    Purpose

    Luciferase reporter plasmid for PE OCT4 enhancer activity

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    Proximal element of hOCT4 promoter (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-Flag-SPRTN-wt

    Plasmid

    #110214

    Purpose

    Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

    Depositor

    Kristijan Ramadan, Ivana Marinovic-Terzic

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N1-HSD17B13-WT-FLAGC

    Plasmid

    #184501

    Purpose

    Expresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)
    

    Use

    Tags

    FLAGC-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-N1-HSD17B11-WT-FLAGC

    Plasmid

    #161903

    Purpose

    Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    

    Use

    Tags

    FLAG-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AICSDP-45: TTN-mEGFP

    Plasmid

    #114412

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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