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pAW-NbVHH05-mCherry

Plasmid

#171572

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Publication

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-mCherry (UBE2J1 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-LAMP1

Plasmid

#168513

Purpose

Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

Depositor

Franck Polleux

Publication

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1 (Lamp1 Rat)

Tags

mEmerald

Expression

Mammalian

Promoter

CAG

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5-FRT-TO-Nsp16-HF

Plasmid

#157725

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Publication

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K5-2xCSTREP

Plasmid

#136154

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K5

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Publication

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-mROCK1-delta3

Plasmid

#101291

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

1-1080 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta2

Plasmid

#101290

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

1-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Publication

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Publication

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Publication

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Publication

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAD

Tags

3xFlag, His, and T7

Expression

Mammalian

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Publication

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCV Small t antigen

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Publication

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Epsin 2 (EPN2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MDH1-miR-181a1-Dusp6

Plasmid

#17849

Depositor

Chang-Zheng Chen

Publication

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

Dusp6-EGFP (Dusp6 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Publication

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pCAGEN-dPspCas13b-NES-mCherry

Plasmid

#233609

Purpose

Expression of catalytically-inactive PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Publication

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dPspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Mutation

H133A/H1058A

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF3228

Plasmid

#144704

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAX (MAX Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0278

Plasmid

#142584

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARID2 (ARID2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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