We narrowed to 80,471 results for: MYC

1. pcDNA3-mCherry-mCherry-FLARE-AKAR

   Plasmid

   #123333

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-AKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-EcRT-GAL-Kan

   Plasmid

   #232092

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. ScSpe1 pET-20b(+)

   Plasmid

   #117145

   Purpose

   Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

   Depositor

   Markus Ralser

   Article

   Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

   Insert

   SPE1
   

   Tags

   Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ss-SNAP-TMD-NS3-Gal4

   Plasmid

   #112279

   Purpose

   Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.

   Depositor

   John Ngo

   Article

   Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

   Insert

   SNAP-TMD-NS3-Gal4
   

   Tags

   AU1 (N term on NS3) and HA (C term on NS3)

   Expression

   Mammalian

   Mutation

   NS3 mutant T54A

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-MTOR-T1977R

   Plasmid

   #69011

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-T1977R (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Threonine 1977 to Arginine

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-AktAR

   Plasmid

   #61624

   Purpose

   FRET-based biosensor for monitoring Akt activity

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR (AKT1 Synthetic, Human, Budding Yeast)
   

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-MTOR-F1888L

   Plasmid

   #69010

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-F1888L (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Phenylalanine 1888 to Leucine

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pYL236

   Plasmid

   #173184

   Purpose

   CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR2.1-promoter-mIL-6

    Plasmid

    #109296

    Purpose

    IL-6 promoter reporter construct

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Expression

    Mammalian

    Mutation

    CMV promoter in pEGFP-N1 replaced with murine IL-…

    Promoter

    murine IL-6 promoter

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid

    #136405

    Purpose

    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    BbsI restriction site at position 437 was removed…

    Promoter

    U6, PDK

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Tags

    His6

    Expression

    Yeast

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

    Plasmid

    #123346

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-CKAR
    

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-MED9

    Plasmid

    #15407

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Expression

    Bacterial and Yeast

    Promoter

    pADH1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDN-T2dGZmxh

    Plasmid

    #44552

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Promoter

    PTETREG

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyG2_zeo

    Plasmid

    #184913

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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