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Sema3c(L)-Fc-His

Plasmid

#72141

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn5.a-Fc-His

Plasmid

#72107

Purpose

Expresses the entire Netrin 5, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5.a (Ntn5 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-MtPylRS (human-opti)

Plasmid

#164080

Purpose

To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtPylRS-478(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MfBulKRS (human-opti)

Plasmid

#164196

Purpose

To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

Mf BulKRS(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Y269A;Y347F

Promoter

CMV

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Neo1.h-AP-His

Plasmid

#71970

Purpose

Expresses the extracellular region of the Neogenin 1, isoform h protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.h (Neo1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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HA-POMP

Plasmid

#86765

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human POMP (POMP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4-2 WW2

Plasmid

#111883

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: NEDD4-2 WW2 fused to C-term…

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf166

Plasmid

#12854

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jon Yewdell, Jack Bennink

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf166

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pAM-AAV-mSncg-1.03kb-EGFP

Plasmid

#153164

Purpose

Truncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates gene expression in mammalian retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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PF3D7_0606800-bio

Plasmid

#47790

Purpose

Expresses enzymatically monobiotinylated full-length PF3D7_0606800 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised PF3D7_0606800

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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Sema4b-AP-His

Plasmid

#72029

Purpose

Expresses the extracellular region of the Sema4B protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4b (Sema4b Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn3-Fc-His

Plasmid

#72105

Purpose

Expresses the entire Netrin 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn3 (Ntn3 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pOCC94

Plasmid

#118912

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette

Use

Tags

MBP, cleavable with 3C protease and eGFP-HIS6

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-Fc-His

Plasmid

#72142

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pOCC254

Plasmid

#118889

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

HIS6-MBP, cleavable with 3C protease; mCherry, cl…

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema4g-AP-His

Plasmid

#72034

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCK-D390TN(UniProt)-FLAG

Plasmid

#164693

Purpose

Express N-terminally truncated DroshaTN-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated transdominant negative Drosha (DROSHA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD-CMV-STAT5A-DNL-CMV-GFP

Plasmid

#83253

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5A (Stat5a Mouse)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

C-terminal deletion of 351 nucleotides (dominant …

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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