We narrowed to 7,531 results for: Ski;

1. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Mutation

   7mer insertion in the AAV2 cap

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBrain-GFP-FKBP-TACC3-shTACC3

   Plasmid

   #59354

   Purpose

   Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   EGFP and FKBP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBrain-GFP-TACC3KDP-shTACC3

   Plasmid

   #59356

   Purpose

   Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV CAG ChR2 E123T T159C 2A tDimer

   Plasmid

   #85399

   Purpose

   High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   CAG and ribosomal skip sequence 2A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MigR1-Flag-TRIB3

   Plasmid

   #131159

   Purpose

   Retroviral expression of flag-tagged mouse TRIB3

   Depositor

   Warren Pear, Karen Keeshan

   Article

   Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

   Insert

   Trib3 (Trib3 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 T7 Jip1(delta JBD)

   Plasmid

   #51767

   Purpose

   " JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   deleted 126-263

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PASK gRNA (BRDN0001145672)

   Plasmid

   #76203

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PASK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PASK (PASK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUMO1-del

   Plasmid

   #228950

   Purpose

   express the full-length human SAE1:SAE2 heterodimer ( where SAE1 is untagged and SAE2 contains an N-terminal tag : MGSSHHHHHHSQDADLNSRVD, derivated from the Addgene plasmid #52258 pSUMO1)

   Depositor

   Marcin Suskiewicz

   Inserts

   UBA 2 (UBA2 Human)
   Aos1 (SAE1 Human)
   

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Promoter

   RBS and T7-lac

   Available Since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gal4-HSF4b K294R

    Plasmid

    #118359

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    K294R

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PASK gRNA (BRDN0001149035)

    Plasmid

    #76204

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PASK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PASK (PASK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PASK gRNA (BRDN0001148005)

    Plasmid

    #76205

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PASK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PASK (PASK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcdna3 T7 Jip1b(471-707)(C478S and C699T)

    Plasmid

    #52132

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-470 and changed C478S and C6…

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcdna3 T7 Jip1b(282-470)

    Plasmid

    #52125

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-281 and 471-707

    Available Since

    April 18, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcdna3 T7Jip1(282-470)

    Plasmid

    #51703

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 1-281 and 471-660

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. LP725

    Plasmid

    #90182

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 561-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa residues 561-1826

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LP399

    Plasmid

    #90176

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1-1289

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LP711

    Plasmid

    #90180

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1000-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa residues 1000-1826

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LP697

    Plasmid

    #90178

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1650-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1650-1826

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LP712

    Plasmid

    #90181

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 840-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa residues 840-1826

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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