We narrowed to 125,963 results for: CIL

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Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B2

Plasmid

#36942

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B2 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-Myc-IRES-GFP

Plasmid

#35396

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pLv-CMV-MyoD

Plasmid

#26808

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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PKC beta I CAT

Plasmid

#16380

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-671

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Oct4

Plasmid

#19766

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-mScarlet-WPRE

Plasmid

#99280

Purpose

Can be used to generate AAV virus that will express mScarlet in the presence of Cre

Depositor

Rylan Larsen

Insert

mScarlet

Use

AAV and Cre/Lox

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc3-L11

Plasmid

#20936

Depositor

Yue Xiong

Article

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Ribosomal protein L11 (RPL11 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Lysosomal-METRIQ

Plasmid

#135401

Purpose

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

DNAse II alpha (DNASE2 Human)

Use

Lentiviral

Tags

T2A, mCherry, and sfGFP

Expression

Mammalian

Mutation

Single-nucleotide polymorphism C432T

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-DRGFP

Plasmid

#113193

Purpose

DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

Depositor

Adam Karpf

Insert

DR-GFP

Use

Donor construct with t2a-hygromycin for gene trap…

Expression

Mammalian

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Tags

ECFP and YPET

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-tdTomato-WPRE-pGHpA

Plasmid

#67527

Purpose

"Expresses the fluorescent protein tdTomato"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

tandem dimer tomato

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPb LAP

Plasmid

#66979

Purpose

Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP beta (Cebpb Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-Jaws-KGC-GFP-ER2

Plasmid

#65015

Purpose

AAV-mediated expression of Jaws under the CaMKII promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
Mfn1(K88T)-10xmyc

Plasmid

#26050

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Tags

10xMyc

Expression

Mammalian

Mutation

K88T GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only