We narrowed to 18,937 results for: Tec

1. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sfGFP-SasG(3G)-mCherry

   Plasmid

   #206945

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (3G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (3G)-mCherry
   

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLINE1ΔORF2-scFvFc

   Plasmid

   #206021

   Purpose

   The ORF2 sequence was removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
   

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLINE1ΔORF1-2-scFvFc

   Plasmid

   #206022

   Purpose

   The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sfGFP-SasG(7G)-mCherry

   Plasmid

   #206949

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (7G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (7G)-mCherry
   

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sfGFP-SasG(6G)-mCherry

   Plasmid

   #206948

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (6G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (6G)-mCherry
   

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sfGFP-SasG(5G)-mCherry

   Plasmid

   #206947

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (5G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (5G)-mCherry
   

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sfGFP-SasG(4G)-mCherry

    Plasmid

    #206946

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (4G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (4G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sfGFP-SasG(2G)-mCherry

    Plasmid

    #206944

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (2G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (2G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PET-evoSpCas9-NLS-6xHis

    Plasmid

    #207389

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXS-ATPIF1 E55A

    Plasmid

    #204704

    Purpose

    expresses ATPIF1 E55A in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Expression

    Mammalian

    Mutation

    E55A

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Shim_ProRS_TP_GFP_pK7FWG2

    Plasmid

    #202651

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic ProRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Shim_ThrRS_TP_GFP_pK7FWG2

    Plasmid

    #202652

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic ThrRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Shim_TrpRS_TP_GFP_pK7FWG2

    Plasmid

    #202653

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic TrpRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Shim_AspRS_TP_GFP_pK7FWG2

    Plasmid

    #202647

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic AspRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. Shim_GlyRS_TP_GFP_pK7FWG2

    Plasmid

    #202648

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic GlyRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Shim_AsnRS_TP_GFP_pK7FWG2

    Plasmid

    #202649

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic AsnRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Shim_MetRS_TP_GFP_pK7FWG2

    Plasmid

    #202650

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic MetRS
    

    Tags

    Green Florescent Protien

    Expression

    Plant

    Available Since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge