We narrowed to 13,692 results for: Egf

1. ER-G-Ca-FLITS-KDEL

   Plasmid

   #191464

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   ER and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-U6-sgRNA_SRD5A1-mcherry

   Plasmid

   #159780

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-mROCK1-delta7

   Plasmid

   #101295

   Purpose

   Mammalian expression of Rock1-GFP for the study of localisation signaling.

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (Rock1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   727-1354 aa

   Promoter

   CMV

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. rat APOBEC1 E63A

   Plasmid

   #112861

   Purpose

   plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Glutamic acid 63 to Alanine

   Promoter

   CMV promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA-T-intron-G

   Plasmid

   #36887

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (i.e., ATG, start codon as a first exon)
   GFP
   beta-globin intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides before nucleotide 275 and ins…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. SH4-FRB-mRFP

   Plasmid

   #100741

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   Fyn
   

   Use

   Tags

   FRB and mRFP

   Expression

   Mammalian

   Mutation

   SH4 domain only

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-WingGFP-tan

   Plasmid

   #83473

   Purpose

   EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   WingGFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Hsp70 and enhancer from D. melanogaster yellow ge…

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA-G-intron-T

   Plasmid

   #36885

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 PRKCA

   Plasmid

   #67115

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PRKCA Protein kinase C alpha type (PRKCA Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAID1.2-CMV-NGFP-mAID

    Plasmid

    #140605

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AB.pCCL.sin.cPPT.GFP.miR-617.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85921

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-617 Sensor (MIR617 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-Xl-VSP1-GFP

    Plasmid

    #51883

    Purpose

    expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHB4

    Plasmid

    #53462

    Purpose

    This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

    Depositor

    Elizabeth Conibear

    Article

    Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

    Insert

    yeGFP-Snc1-Suc2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBEAST2-sfGFP

    Plasmid

    #126575

    Purpose

    Strong constitutive expression of super-folder GFP for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB.pCCL.sin.cPPT.U6.miR-182-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46596

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-182 Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. CAV1-D67G-HA

    Plasmid

    #27706

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutant D67G

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. sspa-GCaMP6s

    Plasmid

    #67559

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAID1.2-CMV-NmScarlet-mAID

    Plasmid

    #140618

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AB.pCCL.sin.cPPT.U6.miR-181b-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46595

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-181b Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pFU-iMVIIA-PE

    Plasmid

    #24146

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Feb 7. ():.

    Insert

    omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP-IRES-TetR-KRAB (PDGFRB Conus magus, Aequorea victoria, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AB.pCCL.sin.cPPT.GFP.miR-639.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85923

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-639 Sensor (MIR639 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pILGFP9E3

    Plasmid

    #185842

    Purpose

    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pmyc-GFP-TNRC6A-(1-1076)

    Plasmid

    #42014

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-1076

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FAM19A1-V5

    Plasmid

    #184337

    Purpose

    Expression of V5-tagged mouse FAM19A1

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    FAM19A1 (Tafa1 Mouse)
    

    Use

    Tags

    V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-mROCK1-delta5

    Plasmid

    #101293

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    375-727 aa

    Promoter

    CMV

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p3.2mar-GFP

    Plasmid

    #63694

    Purpose

    3.2 kb marine armor plate enhancer driving GFP reporter

    Depositor

    David Kingsley

    Article

    O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

    Insert

    3.2 kb marine stickleback armor plate enhancer
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hsp70

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCellFree_G03 PPARG

    Plasmid

    #67099

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PPARG (PPARG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. CRISPRmTmG2

    Plasmid

    #69992

    Purpose

    The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA that targets near LoxP sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Avr2-GFP pZK538

    Plasmid

    #118011

    Purpose

    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

    Depositor

    FLW Takken

    Article

    et al Bio-protocol 8(24): e3114.

    Inserts

    dspAvr2
    mCherry
    

    Use

    Tags

    EGFP and HDEL

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge