We narrowed to 11,501 results for: ARIA

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pGEX-6P-1-gp78 RING

Plasmid

#185353

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 cytoplasmic domain (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codon after aa 393

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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B-HypaR-SpCas9

Plasmid

#126764

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A; N692A; M694A; Q695A; H698A; amino acids 10…

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(C)

Plasmid

#206142

Purpose

Expresses the rs27895-C allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 429-611

Plasmid

#185354

Purpose

GST fusion protein for quantification of gp78 levels

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

aa 429-611 only

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 292-410 5E

Plasmid

#185332

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutations R382E/Q383E/K390E…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 292-410 A397D

Plasmid

#185333

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutation A379D

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRgp78

Plasmid

#185344

Purpose

Expression of soluble G2BR, amino acids 575-600 of human gp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 aa 574-600 (G2BR) (AMFR Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 575-600 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST-AUP1 379-410:Ube2G2

Plasmid

#185335

Purpose

Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

Plasmid

#148520

Purpose

Mammalian Expression of HseIF4G1-L640AV646A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1-L640AV646A (EIF4G1 Human)

Expression

Mammalian

Mutation

one non silent mutation M432V (natural variant is…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

Plasmid

#148521

Purpose

Mammalian Expression of HseIF4G1-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1-LIVVtoAAAA (EIF4G1 Human)

Expression

Mammalian

Mutation

one non silent mutation M432V (natural variant is…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

Plasmid

#148522

Purpose

Mammalian Expression of HseIF4G1-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1-Y619AL624AL625A (EIF4G1 Human)

Expression

Mammalian

Mutation

one non silent mutation M432V (natural variant is…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

Plasmid

#165085

Purpose

gRNA 1 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

Plasmid

#165086

Purpose

gRNA 2 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

Plasmid

#169468

Purpose

Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

Plasmid

#169466

Purpose

Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSico_U6-PGC1a1 sgRNA

Plasmid

#165425

Purpose

Expression of gRNA against human PGC-1a variant 1

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against PGC-1a variant 1 (PPARGC1A Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only
pPGK-T7/2-CD44v2-10 (-cyt)

Plasmid

#137825

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region - N213S on the CD44 re…

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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