We narrowed to 8,677 results for: sgrna

1. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

   Plasmid

   #87906

   Purpose

   sgRNA targeting GFP

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgGFP1, sgGFP2, sgGFP3
   

   Expression

   Mammalian

   Promoter

   H1, U6, 7SK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSECC-sgTom

   Plasmid

   #138661

   Purpose

   Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgTomato
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. iMAP-61

   Plasmid

   #187460

   Purpose

   gRNA array of iMAP-61

   Depositor

   Tian Chi

   Article

   Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

   Insert

   sgRNA array
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   modified U6

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1221

   Plasmid

   #119260

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Blast-ultraID-LMNB1

   Plasmid

   #207775

   Purpose

   Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLV-hIP-dCas9-BSD

   Plasmid

   #183231

   Purpose

   Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   Human insulin promoter

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

   Plasmid

   #157986

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330 Ctnnb1.2

    Plasmid

    #59912

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

    Plasmid

    #149278

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP1104

    Plasmid

    #119248

    Purpose

    knockdown pqsC in P. aeruginosa

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA pqsC (P. aeruginosa)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

    Plasmid

    #201685

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

    Plasmid

    #201686

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1 (V106W)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

    Plasmid

    #218524

    Purpose

    sgRNA targeting human KIAA0141/DELE1

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    DELE1 (DELE1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

    Plasmid

    #218525

    Purpose

    sgRNA targeting human KIAA0141/DELE1

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    DELE1 (DELE1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pODN-mNG-ECT2

    Plasmid

    #183835

    Purpose

    Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBLO2 casX

    Plasmid

    #123122

    Purpose

    E. coli expression vector for CasX sgRNA

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX guide RNA
    

    Use

    CRISPR

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJET1.2-U3

    Plasmid

    #173156

    Purpose

    Single guide RNA cassette under U3 promoter

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Insert

    sgRNA cassette under U3 promoter
    

    Expression

    Plant

    Promoter

    promoter region of the U3C snRNA gene (Marshallsa…

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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