We narrowed to 15,542 results for: LEA

1. kiCAP-AAV-MyoAAV4C

   Plasmid

   #224450

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYTSM insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-MyoAAV3C

   Plasmid

   #224444

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYSSV insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. kiCAP-AAV-MyoAAV3B

   Plasmid

   #224443

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYSGL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV40-EF1a-hBCL10-SO

   Plasmid

   #75256

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   Promoter

   pMSCV-LTRs, EF1a

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

   Plasmid

   #217804

   Purpose

   Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-linker-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

   Plasmid

   #208957

   Purpose

   A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pelB-MBP-BB0238-ss

   Plasmid

   #137046

   Purpose

   E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature BB0238: lacks the BB0238 signal peptide

   Promoter

   T7lac

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY0310 TP901-1 Integrase

   Plasmid

   #179110

   Purpose

   TP901-1 Integrase expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   TP901-1 Integrase
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-DroshaS300E/S302D

   Plasmid

   #62531

   Purpose

   Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 300 to glutamic acid and serine 3…

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDule-3-nitroTyrosine (A7)

    Plasmid

    #174078

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid

    #208954

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

    Plasmid

    #176278

    Purpose

    Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-7 asyn G51D

    Plasmid

    #105747

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

    Insert

    alpha-synuclein G51D (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G51D

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid

    #122085

    Purpose

    Express M.EcoGII fused to CENPC with destabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of CENPC

    Expression

    Bacterial and Mammalian

    Mutation

    L341F, R930L (see comments below)

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2-SBECN1W

    Plasmid

    #107937

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    mouse Beclin-1 (BECN1) (Becn1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-α-Syn-nYFP

    Plasmid

    #92203

    Purpose

    Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Retroviral

    Tags

    YFP(1-155)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cherry-HA-KCC2

    Plasmid

    #104077

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Use

    Tags

    mCherry-HA tag

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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