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pMOD_A1610

Plasmid

#91038

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT5

Plasmid

#53560

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pTaCas9_6

Plasmid

#91169

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRV DYRK2

Plasmid

#12568

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ACR_Trypsin

Plasmid

#110042

Purpose

Protein expression and purification of ACR_Trypsin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ACR_Trypsin (ACR Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPN217

Plasmid

#91676

Purpose

Express sgRNA targeting human TLE1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TLE1 (TLE1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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mouse Cavin-1

Plasmid

#50441

Purpose

expresses mouse Cavin-1 in mammalian cells

Depositor

Paul Pilch

Article

Liu et al J Biol Chem. 2008 Feb 15;283(7):4314-22.

Insert

Cavin-1 (Cavin1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
PhyB-mCherry-SIK1

Plasmid

#51577

Purpose

Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SIK1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTC365

Plasmid

#91214

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MBD3L2

Plasmid

#106332

Purpose

All-in-one lentivector with tet-inducible MBD3L2

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-CHAD

Plasmid

#127793

Purpose

Expresses the CHAD domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF-CHAD (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 201-433)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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p6896 PHAGE-P CMVT N-HA-hE6AP II wt

Plasmid

#37603

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II wt (UBE3A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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MTK3_020

Plasmid

#123735

Purpose

Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

ERT2-fkbp-spdCa9(205-1368)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5(N140A)

Plasmid

#157806

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5(N140A)

Tags

His6 and MBP

Expression

Bacterial

Mutation

N140A

Promoter

pTAC

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SNF7

Plasmid

#51574

Purpose

Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SNF7 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Cx43-7

Plasmid

#57787

Purpose

Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Cx43

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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y2

Plasmid

#53586

Purpose

Produces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

Insert

lcyE (LUT2 Mustard Weed)

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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