We narrowed to 1,197 results for: Aspa

1. pMT-DREDD-V5-His

   Plasmid

   #63749

   Purpose

   Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
   

   Use

   Tags

   V5-6XHis

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET FLIPE-100u

   Plasmid

   #13539

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pfMDH_D230A

   Plasmid

   #67725

   Purpose

   Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed aspartate 230 to alanine

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pfMDH_N191A

   Plasmid

   #67724

   Purpose

   Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 191 to alanine

   Promoter

   T7

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. piHGpd/G170D

   Plasmid

   #1113

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

   Insert

   HSP82 (HSP82 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   HSP82 with glycine 170 changed to aspartic acid; …

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pJExpress414_GmPOPB-D661A

   Plasmid

   #92236

   Purpose

   Express D661A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-D661A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Aspartate 661 to Alanine

   Promoter

   T7 promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS406 ATG9-GFP S122D

   Plasmid

   #114667

   Purpose

   Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   changed serine 122 to aspartic acid

   Promoter

   ATG9

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-fishRab8A[T22N]

   Plasmid

   #24906

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   fishRab8A
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Changed Threonine 22 to Asparagine

   Promoter

   Available since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pJK571

   Plasmid

   #75379

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Gene synthesis

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glycine-209 to aspartate and synthetic ge…

   Promoter

   n/a

   Available since

   Aug. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR-fishRab8B[T22N]

    Plasmid

    #24909

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    fishRab8B
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Changed Threonine 22 to Asparagine

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCAGGS-FLAG-BubR1-D911A

    Plasmid

    #200815

    Purpose

    expresses BubR1-D911A in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Change Aspartic acid 911 to Alanine

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-3C-hTspan12LEL(118-218)_pAcGP67a

    Plasmid

    #216383

    Purpose

    Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Tetraspanin-12 (TSPAN12 Human)
    

    Use

    Baculovirus

    Tags

    GP64 signal sequence-MBP-3C

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FGFR2 N549H 3xFlag

    Plasmid

    #110106

    Purpose

    expresses FGFR2 with a single amino acid change in mammalian cells

    Depositor

    Martin Fernandez-Zapico

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mutated Asparagine 549 to Histidine (N549H)

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FGFR2 N549K 3xFlag

    Plasmid

    #110107

    Purpose

    expresses FGFR2 with a single amino acid change in mammalian cells

    Depositor

    Martin Fernandez-Zapico

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mutated Asparagine 549 to Lysine (N549K)

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJExpress414_GmPOPB-H698N

    Plasmid

    #92242

    Purpose

    Express H698N mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-H698N mutant
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Histidine 698 to Asparagine

    Promoter

    T7 promoter

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK340

    Plasmid

    #71837

    Purpose

    Cloned region contains Acetobacter aceti 1023 purE(H59N) gene

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine

    Promoter

    lac

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK346

    Plasmid

    #71836

    Purpose

    Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to aspartate

    Promoter

    lac

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSKB2-TRexR90D

    Plasmid

    #31350

    Depositor

    Clara Kielkopf

    Article

    McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

    Insert

    Thermus aquaticus Redox Sensing Repressor (R90D)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Arginine 90 to Aspartic Acid

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET-21a(+) SurE D8A

    Plasmid

    #12685

    Depositor

    SeWon Suh

    Article

    Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

    Insert

    SurE (TM1662 )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Aspartate 8 to Alanine

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only