Addgene

We narrowed to 1,174 results for: Aspa

Showing: 741 - 760 of 1174 results

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pJK346

Plasmid

#71836

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

lac

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pfMDH_N300S

Plasmid

#67729

Purpose

Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 300 to serine

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pSKB2-TRexR90D

Plasmid

#31350

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor (R90D)

Use

Tags

His

Expression

Bacterial

Mutation

changed Arginine 90 to Aspartic Acid

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pET-21a(+) SurE D8A

Plasmid

#12685

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE (TM1662 )

Use

Tags

His

Expression

Bacterial

Mutation

changed Aspartate 8 to Alanine

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR2B

Plasmid

#17925

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl D-aspartate receptor 2B (Grin2b Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Sth1Cas9n cytosine base editor

Plasmid

#194209

Purpose

Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

Sth1Cas9-D9A nickase

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Changed Aspartate 9 to Alanine.

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR2P_P474S_P493N

Plasmid

#198695

Purpose

Expression of human FXR2 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR2P (FXR2 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Changed proline 474 to serine and proline 493 asp…

Promoter

T7

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Dead-Laconic/pcDNA3.1(-)

Plasmid

#118627

Purpose

To express in mammalian cells a lactate FRET sensor negative control.

Depositor

Luis Felipe Barros

Article

Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

Insert

Dead-Laconic

Use

Tags

Expression

Mammalian

Mutation

Change Histidine 151 to Aspartic Acid of Escheric…

Promoter

CMV Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET28sumo-hRig-I DCARDS

Plasmid

#210828

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

Insert

hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)

Use

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

Plasmid

#130816

Purpose

Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bJun-NFAST-NLS-DEVDG-mCherry-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12aD156R

Plasmid

#164659

Purpose

Bacterial expression of mutated LbCas12aD156R using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a-D156R

Use

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Mutation

changed Aspartic Acid 156 to Arginine

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-wGBD-mTurquoise2-wGBD

Plasmid

#176136

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-100u

Plasmid

#13543

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pME18S-3HA-mULK1(K46N)

Plasmid

#22897

Depositor

Masaaki Muramatsu

Article

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 46 to Aspargine

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only