We narrowed to 10,449 results for: T7

1. pcDNA3.1-(empty)-TAG

   Plasmid

   #138209

   Purpose

   To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Type

   Empty backbone

   Use

   T7 transcription

   Tags

   TAG sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter, T7 Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-pelB

   Plasmid

   #157740

   Purpose

   empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   PelB signal sequence and possible His6-tag (adds …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAM

   Plasmid

   #45174

   Depositor

   Jumi Shin

   Article

   Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

   Type

   Empty backbone

   Use

   Tags

   6xHis and 6xHis/Thrombin/T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pAB150

   Plasmid

   #101661

   Purpose

   Opto-T7RNAP*(563)

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7RNAP

   Plasmid

   #170101

   Purpose

   expresses T7 RNAP

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   T7-RNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJL1-FETCH1

   Plasmid

   #210644

   Purpose

   In vitro expression of FETCH reporter from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

   Insert

   FETCH1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p7XNH3

   Plasmid

   #47064

   Purpose

   FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTAT2.2

   Plasmid

   #35621

   Depositor

   Steven Dowdy

   Article

   Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

   Type

   Empty backbone

   Use

   Tags

   6xHIS, T7 tag, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAB202

    Plasmid

    #101674

    Purpose

    Opto-T7RNAP(563)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP(563)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmVirD2-GFP

    Plasmid

    #90315

    Purpose

    mVirD2-GFP expression plasmid

    Depositor

    Bjorn Hogberg

    Article

    Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

    Insert

    A. tumefaciens VirD2 from aa 1 to 204 - GFP
    

    Use

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7/T5 IPTG inducible

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p7XC3H

    Plasmid

    #47065

    Purpose

    FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJL1-FETCH1-StrepII

    Plasmid

    #210648

    Purpose

    In vitro expression of FETCH reporter from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

    Insert

    FETCH1
    

    Use

    Synthetic Biology

    Tags

    Strep-TagII

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIK86

    Plasmid

    #47933

    Purpose

    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

    Depositor

    Iskra Katic

    Article

    Katic et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR and Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAT3

    Plasmid

    #112589

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAT2

    Plasmid

    #112588

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAT4

    Plasmid

    #112585

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAB147

    Plasmid

    #101662

    Purpose

    Opto-T7RNAP*(302)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(302)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAT

    Plasmid

    #112582

    Purpose

    Used for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAT5

    Plasmid

    #112586

    Purpose

    Used for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge