We narrowed to 3,177 results for: pcdna3 1

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pcDNA3.1 HisC EXO1b

Plasmid

#68268

Purpose

Mammalian expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Tags

His

Expression

Mammalian

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-DEST-MatryoshCaMP6s

Plasmid

#100025

Purpose

Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

V5 Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-Scramble

Plasmid

#175014

Purpose

Negative-control construct for Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-Scramble

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(-)-Fc

Plasmid

#59313

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(+)-Fc

Plasmid

#59314

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2-NES

Plasmid

#175011

Purpose

Cytosol-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2-NLS

Plasmid

#175012

Purpose

Nuclear-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AktAR

Plasmid

#181856

Purpose

Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AktAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-FL

Plasmid

#105609

Purpose

transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR2

Plasmid

#175013

Purpose

Plasma membrane-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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hJAM-A deltaCT pcDNA3.1

Plasmid

#70074

Purpose

Expresses human junctional adhesion molecule-A amino acids 1-260 in mammalian cells

Depositor

Terence Dermody

Article

Danthi et al J Virol. 2006 Feb;80(3):1261-70.

Insert

junctional adhesion molecule-A (F11R Human)

Expression

Mammalian

Mutation

deleted C-terminal cytoplasmic tail (amino acids …

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FLAG-Rgs14

Plasmid

#236396

Purpose

Expresses FLAG-Rgs14 in mammalian cells

Depositor

John Hepler

Article

Shu et al Cell Signal. 2007 Jan;19(1):163-76. doi:

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGCC2-FLAG

Plasmid

#214166

Purpose

Transient expression of GCC2

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

GCC2 (Gcc2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585_Y167F

Plasmid

#174392

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Mutation

Tyr 167 changed to Phe

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.S61P

Plasmid

#105048

Purpose

Overexpression of APOBEC3C S61P mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

serine 61 to proline

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.D99E

Plasmid

#105049

Purpose

Overexpression of APOBEC3C D99E mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

Aspartate 99 to glutamate

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.DS-EP

Plasmid

#105050

Purpose

Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

S61P, D99E

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.C97S

Plasmid

#105051

Purpose

Overexpression of APOBEC3C C97S mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Tags

HA tag

Expression

Mammalian

Mutation

cysteine 97 to serine

Promoter

CMV

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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