Addgene

We narrowed to 21,567 results for: ESC;

Showing: 7581 - 7600 of 21567 results

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pMH062

Plasmid

#225537

Purpose

Expresses IS621 in insect cells

Depositor

Hiroshi Nishimasu

Article

Hiraizumi et al Nature. 2024 Jun;630(8018):994-1002. doi

Insert

IS621

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+_NLS-Tir1-HA

Plasmid

#117716

Purpose

mRNA synthesis of NLS-TIR1-HA for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

codon-optimized TIR1 for murine expression

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-CaURA3

Plasmid

#44877

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-moxMaple3

Plasmid

#120871

Purpose

Bacterial expression of moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Use

Tags

His

Expression

Bacterial

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Promoter

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K-CAAX

Plasmid

#41644

Purpose

Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-CAAX

Use

Tags

CAAX box

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pAGD236

Plasmid

#219497

Purpose

pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

sfGFP, Eco1 msr, Eco1 RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

ITPKA

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn

Plasmid

#183577

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet-On

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP713-T2A-HO1

Plasmid

#197246

Purpose

Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP680

Plasmid

#197203

Purpose

Expresses the protein of miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 PCNA GFP

Plasmid

#105937

Purpose

in vitro transcription, cell cycle nuclear marker

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

PCNA (PCNA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMDICI

Plasmid

#51652

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

CmR

Use

Genetic modification

Tags

Expression

Mutation

Promoter

unknown

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only
VSVGwt-SBP-EGFP_puromycin

Plasmid

#65302

Purpose

VSVG reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1

Plasmid

#84451

Purpose

shuttle plasmid for sarA P1-dsRED expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

DsRed.T3(DNT)

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAJM.715

Plasmid

#108516

Purpose

PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTac-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pNB768

Plasmid

#60139

Purpose

Green click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-UtrCH

Plasmid

#98823

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

UtrCH

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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