166,289 results

1. CMV-ER-LAR-GECO1

   Plasmid

   #61244

   Purpose

   Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1
   

   Tags

   ER-retention sequence: KDEL and ER-targeting sequ…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: V51W/I113V/N35…

   Promoter

   CMV

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

   Plasmid

   #128604

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7II-2N4Rtau

   Plasmid

   #177653

   Purpose

   Recombinant protein synthesis of human Tau

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   Tau (2N4R) (MAPT Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4 Smad4

   Plasmid

   #16483

   Depositor

   Bert Vogelstein

   Article

   Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

   Insert

   Smad4 (SMAD4 Human)
   

   Tags

   Double HA

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pJT301 BxBi Lenti pEF-attB LSR GFP

   Plasmid

   #184944

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   BxBi Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ub-G76V-GFP

   Plasmid

   #11941

   Depositor

   Nico Dantuma

   Article

   Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

   Insert

   Ub-G76V-GFP
   

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Floxed-STOP mCherry

   Plasmid

   #122963

   Purpose

   mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_Floxed-STOP mCherry
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Tags

   GFP11x7

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRU295

    Plasmid

    #167694

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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