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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Promoter

UbC

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEvol-pAcFRS.2.t1

Plasmid

#73544

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.2.t1
pAcFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-NES reporter (pDN160)

Plasmid

#72660

Purpose

NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a SnoopTag-mEGFP-SpyTag

Plasmid

#72325

Purpose

Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-mEGFP-SpyTag

Tags

N-terminal His6 tag

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pST44

Plasmid

#64007

Purpose

polycistronic cloning vector for pST44 plasmid suite

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pT040

Plasmid

#67640

Purpose

URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Insert

single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfrbeta-EGFPN

Plasmid

#66790

Purpose

Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRbeta (Pdgfrb Mouse)

Tags

C-term EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Promoter

EF1-Alpha

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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PGK-AAVS1ZFNL

Plasmid

#60916

Purpose

encodes zinc finger nuclease specific to AAVS1 locus (left)

Depositor

Paul Gadue

Article

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

AAVS1-ZFNL

Promoter

PGK

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAXipi

Plasmid

#53287

Purpose

Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUltra-puro-RTTA3

Plasmid

#58750

Purpose

silence doxycyclin inducible pUltra-dox

Depositor

Yildirim Dogan, Kitai Kim

Insert

Reverse TET-Transactivator 3

Use

Lentiviral

Expression

Mammalian

Mutation

loxP5171

Promoter

Ubiquitin C promoter

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAdSh.PGK.Cas9

Plasmid

#58253

Purpose

Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

Human codon-optimized S. pyogenes Cas9

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Sequestosome1-N-18

Plasmid

#55132

Purpose

Localization: Membrane Scaffold, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Sequestosome1 (SQSTM1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-Sec5

Plasmid

#53756

Purpose

pEGFP-C3 plasmid that expresses Sec5

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec5 (EXOC2 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only