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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRAU168

Plasmid

#86834

Purpose

bacterial expression of anti-CRISPR protein AcrIIA1

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA1

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSARSF53-165

Plasmid

#61817

Purpose

PhoRadAdC6 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAdC6 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJL030

Plasmid

#53533

Purpose

supplies EtpC required for optimal EtpA expression on compatible plasmid

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpC

Use

Pacyc184

Expression

Bacterial

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-5bHA

Plasmid

#18034

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5b (Wnt5b Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMT2 GATA1

Plasmid

#13626

Depositor

Anjana Rao

Article

Monticelli et al J Biol Chem. 2004 Aug 27. 279(35):36210-

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pGL0_7 [pSa ORI]

Plasmid

#198923

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP21

Plasmid

#80675

Purpose

Encodes the synthetic coiled coil peptide SYNZIP21

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP21

Use

Gateway Cloning

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD2-HA-LRR-NEL-C (B3-B4)

Plasmid

#211860

Purpose

CDS for E3-DART ligase sequence in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-HR1bR181A R185A-C (B3-B4)

Plasmid

#211855

Purpose

CDS for mutant HR1b degron in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Mutation

R181A R185A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pL2-G

Plasmid

#137090

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5-QL

Plasmid

#29688

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

RAB5A

Tags

DSRed2

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pEMF037

Plasmid

#157634

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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mEos3.2-MCPA2-C-18

Plasmid

#57469

Purpose

Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

MCPA2 (Capza2 Mouse)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5 Flag-DPC4

Plasmid

#14039

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pDRF1-GW ymVenus

Plasmid

#118454

Purpose

Overexpression of yeast codon-optimized mVenus

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 DN-GSK3-GFP

Plasmid

#29681

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Mutation

K85R and K86R

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pDL1403

Plasmid

#226635

Purpose

This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

Depositor

Andrew Camilli

Article

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Inserts

tfox
qstR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0296

Plasmid

#162305

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, superfolder GFP, no stop codon

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pKS2

Plasmid

#24625

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only

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RTA

Plasmid

#64029

Purpose

Expression of Ricin A Chain (RTA) in E. coli

Depositor

Vern Schramm

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Dec 1;106

Insert

Ricin A Chain

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pmEOS2-VSVG

Plasmid

#98286

Purpose

mammalian expression of VSVG-mEOS2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

VSVG

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-Sapphire

Plasmid

#103976

Purpose

Plasmid encoding Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S72A, Y145F,…

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO4

Plasmid

#24319

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

AGO4 (AGO4 Human)

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
pLZL2386

Plasmid

#48682

Purpose

pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

Depositor

Suzanne Sandmeyer

Article

Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

Insert

PFV Integrase

Tags

His

Expression

Bacterial

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-6His-SUMO-Nsp14

Plasmid

#154956

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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AP-SEMA3A (chick)

Plasmid

#29448

Depositor

Jonathan Raper

Article

Feiner et al Neuron. 1997 Sep . 19(3):539-45.

Insert

AP-SEMA3A (chick) (SEMA3A Chicken)

Tags

AP tagged

Expression

Mammalian

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
mEos4b-Actin-C-18

Plasmid

#57500

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

Actin (ACTB Human)

Tags

mEos4b

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFA6a-A1-FLAG:hphMX4

Plasmid

#134429

Purpose

PCR-based C-terminal A1-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1-FLAG

Use

Pcr-based tagging in yeast

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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