19,660 results

1. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

   Plasmid

   #207863

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
   

   Use

   AAV

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. M31: pHR-SFFVp- TDP-43C::mCherry::SspB

   Plasmid

   #122669

   Purpose

   C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   TDP43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry-SspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 218-414

   Promoter

   SFFV

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pV1524

   Plasmid

   #111431

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CFP-GAI(1-92)

   Plasmid

   #37307

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available Since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-myc3-CUL4A

   Plasmid

   #19951

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CUL4A (cullin 4A) (CUL4A Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPED

   Plasmid

   #162468

   Purpose

   Destination Expression vector for dicot plant prime editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine based sequence o…

   Promoter

   2x35S

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. human STIM1 CFP

   Plasmid

   #19755

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Retroviral

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-PS9

   Plasmid

   #177944

   Purpose

   Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP (ORF1ab )
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBsaBM3

   Plasmid

   #85102

   Purpose

   P450 BM3 variant (A74G/C773S/C810S)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450 BM3
   

   Use

   Cloning vector

   Mutation

   A74G/C773S/C810S

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR SFFVp YFP-PIF-SOS2cat

    Plasmid

    #50851

    Purpose

    Optogenetic Ras activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Inserts

    SOS2cat (SOS2 Human)
    PIF6 1-100
    

    Use

    Lentiviral

    Tags

    YFP

    Mutation

    residues 566–1049

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-HA-UBE3A-IRES-Puro

    Plasmid

    #107349

    Purpose

    Expresses HA tagged UBE3A in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3A (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pFA6a URA3

    Plasmid

    #61924

    Purpose

    Expression of S. cerevisiae URA3 cloned into pFA6a

    Depositor

    Jon Houseley, David Tollervey

    Article

    Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

    Insert

    URA3
    

    Use

    Yeast genomic targeting

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-SOX4

    Plasmid

    #110367

    Purpose

    Mammalian expression of HA-tagged SOX4

    Depositor

    Carlos Moreno

    Insert

    SOX4 (SOX4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Expression

    Mammalian

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3_SARS2_omicron BA.2

    Plasmid

    #183700

    Purpose

    expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (BA.2) (S SARS-CoV-2)
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    c-terminal 18 aa deletion

    Promoter

    CMV

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEmerald-TOMM20-N-10

    Plasmid

    #54282

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mAzurite-C1

    Plasmid

    #54583

    Purpose

    Localization: C1 Cloning Vector, Excitation: 384, Emission: 450

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

    Type

    Empty backbone

    Tags

    mAzurite

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. LZRS-DNMT1

    Plasmid

    #24952

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Retroviral

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. EBFP-C1

    Plasmid

    #54738

    Purpose

    Localization: C1 Cloning Vector, Excitation: 387, Emission: 450

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    EBFP

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge