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pET23a-His-TEV-HaloTag-linker-SpyCatcher3

Plasmid

#235150

Purpose

Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

HaloTag linked to SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-αC

Plasmid

#197650

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-2 αC

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Ser677T substitution

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Tags

Citrine and ECFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKEK3395

Plasmid

#234079

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

YukonOFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA RBS30 mscarlet

Plasmid

#226370

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

mScarlet

Tags

6x Histag

Expression

Bacterial

Promoter

copA promoter (pCopA)

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Plasmid

#198450

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2B

Plasmid

#183325

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2B

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2A

Plasmid

#183324

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046959

Plasmid

#145722

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMM289

Plasmid

#46037

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN E84A/K577M (WRN Human)

Use

Tre, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

E84A/K577M exonuclease and helicase-dead mutant; …

Promoter

CMV2

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMM287

Plasmid

#46035

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN K577M (WRN Human)

Use

Teton, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

K577M helicase-dead mutant; S2G introduced during…

Promoter

CMV2

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_Short_V5-GFP

Plasmid

#221497

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKS17

Plasmid

#24628

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

pdc, adhB, atfA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCLC3sGFP

Plasmid

#52423

Purpose

mammalian expression of rat CLC3 short transcript fused to EGFP

Depositor

Steven Weinman

Article

Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

Insert

CLC3 (Clcn3 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-3xrGBD

Plasmid

#176100

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-b5 ER

Plasmid

#38274

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Cytochrome b5 (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Cyb5a Tranmembrane region from 95 to 134 amino ac…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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lenti GFAABC1D-dCas9-KRAB-MeCP2

Plasmid

#194701

Purpose

Expresses dCas9-KRAB-MeCP2 in Astrocytes

Depositor

Timothy Jarome

Article

Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

Insert

GFAABC1D promoter

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

None

Promoter

GFAABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-TetOn-NLS-mCherry

Plasmid

#193762

Purpose

Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

NLS-mCherry

Use

Lentiviral

Tags

mCherry

Mutation

SV40 NLS fused to mCherry

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Mutation

Rice codon optimized

Promoter

2x35S

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL4-DBD

Plasmid

#138394

Purpose

RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4-DBD

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA mcherry

Plasmid

#167910

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p6610 MSCV-IP N-HAonly TIMP1

Plasmid

#35009

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

TIMP1 (TIMP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pME-lamp1

Plasmid

#109559

Purpose

Multisite gateway vector for 5' tagging with lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p-G4PID-HA

Plasmid

#209639

Purpose

Expression G4PID-HA in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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