We narrowed to 22,434 results for: ESC;

1. pHEA

   Plasmid

   #168297

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

   Insert

   Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
   

   Tags

   6xHis, E-tag, and PelB signal peptide

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mPA-GFP-Farnesyl-5

   Plasmid

   #57133

   Purpose

   Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45565

   Purpose

   Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   I-Sce I Nuclease
   BFP
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGas-miRFP670nano

   Plasmid

   #127441

   Purpose

   G protein alpha-s internally tagged with miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   G protein alpha-s-miRFP670nano
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB1A3-AD011

   Plasmid

   #116852

   Purpose

   Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   mScarletI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-ECFPEYFP

   Plasmid

   #24520

   Depositor

   Liusheng He, Jehonathan Pinthus

   Article

   He et al Cytometry A. 2003 Oct . 55(2):71-85.

   Insert

   ECFP, EYFP
   

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Available Since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pBTR(hCc)

   Plasmid

   #61026

   Purpose

   Expresses horse holocytochrome c in E. coli

   Depositor

   Gary Pielak

   Article

   Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

   Insert

   horse cytochrome c (CYCS Equus*)
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   *see comments

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pGK401red-­rtetTAK8N, L131L

   Plasmid

   #165968

   Purpose

   Expresses rTetTA (K8N, L131L) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   tetR
   

   Use

   Synthetic Biology

   Tags

   three copies of VP16 (VP48)

   Expression

   Yeast

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_NES_HaloTag9

   Plasmid

   #175537

   Purpose

   Strict cytosolic expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   NES-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yomCherry-SpHis5

    Plasmid

    #44841

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mCherry with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomCherry
    

    Expression

    Yeast

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSW071

    Plasmid

    #140037

    Purpose

    PCC 11901 fadD locus KO with a kanamycin resistance cassette

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Kanamycin resistance gene
    

    Use

    Gene knockout in cyanobacteria

    Promoter

    KanR

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLG016 (ApeA)

    Plasmid

    #157894

    Purpose

    Expresses the ApeA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    ApeA (putative HEPN RNase)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRU48

    Plasmid

    #167670

    Purpose

    ENTRY vector for gRNA8 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ateam1.03-nD/nA/pENTR1A

    Plasmid

    #51959

    Purpose

    Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Entry vector

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGST2-TSG101 (2-145)

    Plasmid

    #184810

    Purpose

    Bacterial expression of TSG101 UEV domain (aa 2-145)

    Depositor

    James Hurley

    Article

    Im et al Structure. 2010 Nov 10. 18(11):1536-47.

    Insert

    TSG101 (TSG101 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-145 only

    Promoter

    tac

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. POM43-mTFP1

    Plasmid

    #69853

    Purpose

    N-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1 minus start and stop codon
    

    Expression

    Yeast

    Available Since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU47

    Plasmid

    #167669

    Purpose

    ENTRY vector for gRNA7 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNIR-Fbactin

    Plasmid

    #184677

    Purpose

    NIR-Fb to actin

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fbactin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CIp10 - mTurquoise2 (γmGFP based)

    Plasmid

    #163121

    Purpose

    Expresses in Candida albicans

    Depositor

    Patrick Van Dijck

    Article

    Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

    Insert

    mTurquoise2 - γmGFP based
    

    Use

    Candida albicans expression

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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