We narrowed to 11,806 results for: nsf

1. ST-SBP-mCherry_puromycin

   Plasmid

   #65271

   Purpose

   ST reporter only ( no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-GalT-EGFP

   Plasmid

   #174471

   Purpose

   Express EGFP with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with golgi apparatus-targeting sequence
   

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1290-AAV-dSaCas9

   Plasmid

   #223139

   Purpose

   AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB-Flag-MyoD-E12

   Plasmid

   #25990

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD-E12 Heterodimer Fusion (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Available Since

   Sept. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCHR-inGFPt

   Plasmid

   #60237

   Purpose

   HIV-1 transfer vector that encodes gfp-turbo reporter gene interrupted by intron

   Depositor

   Dmitriy Mazurov

   Article

   Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

   Insert

   inGFPt
   

   Use

   Lentiviral

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-synP-FLEX-splitTVA-EGFP

   Plasmid

   #52474

   Purpose

   Expresses splitTVA-EGFP

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Insert

   splitTVA-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   Synapsin-1

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-SauriCas9-Puro

   Plasmid

   #135965

   Purpose

   Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-Chronos-mT-Sapphire-NRN

   Plasmid

   #149692

   Purpose

   For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

   Depositor

   Robert Malenka

   Article

   Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

   Insert

   Chronos
   

   Use

   AAV

   Tags

   mT-Sapphire

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-Arch-GFP

   Plasmid

   #37810

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Insert

   Arch
   

   Use

   AAV; Adeno-associated virus

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Venus-GalT

    Plasmid

    #11931

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Syn-FLEX-ExSYTE

    Plasmid

    #216263

    Purpose

    Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    ExSYTE P2A EGFP - Rat DTE
    

    Use

    AAV

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM-AAV-mSyn1-EGFP

    Plasmid

    #153187

    Purpose

    Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNHEJ-RPG

    Plasmid

    #85931

    Purpose

    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

    Depositor

    Zhiying Zhang

    Article

    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

    Insert

    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    

    Use

    Dual-reporter surrogate

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV hSyn FLEx-loxp hM4Di-mCherry

    Plasmid

    #161581

    Purpose

    Expression of hM4Di-mCherry in a cre-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-ABE-CT

    Plasmid

    #112876

    Purpose

    AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE-CT
    

    Use

    AAV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_tKiMBI-T2A-caMEK

    Plasmid

    #199578

    Purpose

    Expresses tKiMBI and caMEK in an AAV vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
    constitutively active MEK
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ii-Str_ST-SBP-mCherry

    Plasmid

    #65269

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Luciferase shRNA

    Plasmid

    #225343

    Purpose

    shRNAmir backbone for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    Luciferase shRNA (LOC116160065 Mouse)
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    CBA

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGE668

    Plasmid

    #153233

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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