We narrowed to 10,431 results for: plasmids 101

1. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

   Plasmid

   #111396

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   DTR-EGFP
   

   Use

   AAV

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

   Plasmid

   #223148

   Purpose

   Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

   Plasmid

   #223151

   Purpose

   Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 115-177

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

   Plasmid

   #223141

   Purpose

   Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 115-177

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

   Plasmid

   #223158

   Purpose

   Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-tetO-hOKMS

   Plasmid

   #51543

   Purpose

   Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

   Depositor

   Tarjei Mikkelsen

   Article

   Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

   Insert

   hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_GOPC_WT

   Plasmid

   #82924

   Purpose

   Gateway Donor vector containing GOPC, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   GOPC (GOPC Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB CRIV Gc Spike H6

   Plasmid

   #240163

   Purpose

   Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

   Depositor

   Stephen Graham

   Article

   Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

   Insert

   CRIV Gc spike
   

   Use

   Piggybac

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   contains residues 478-906 only

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_BiVIPe4_Gq_dTomato

   Plasmid

   #213858

   Purpose

   AAV construct expressing HA-hM3Dq-dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   HA-GqDREADD-dTomato
   

   Use

   AAV

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_BiPVe3_Gq_dTomato

    Plasmid

    #213942

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_BiSSTe4_Gq_dTomato

    Plasmid

    #213946

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_BiPVe4_Gq_dTomato

    Plasmid

    #213938

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_BiCHATe27_Gq_dTomato

    Plasmid

    #213832

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_ spike_del19

    Plasmid

    #155297

    Purpose

    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

    Depositor

    Raffaele De Francesco

    Article

    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

    Insert

    SARS-Cov2 spike_deleted (S SARS-Cov2)
    

    Use

    Generation of sars-cov-2-pseudotyped lentiviral p…

    Mutation

    Deletion in C-term 19 aa of spike

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV_BiSSTe10_Gq_dTomato

    Plasmid

    #213817

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_BiLAMP5e3_Gq_dTomato

    Plasmid

    #213916

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR D-TOPO-C3_20

    Plasmid

    #60266

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    CDKN1C enhancer (CDKN1C Human)
    

    Use

    Entry vector

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR D-TOPO-C2_10

    Plasmid

    #60250

    Purpose

    This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    LINGO2 inactive enhancer (LINGO2 Human)
    

    Use

    Entry vector

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-HDRT-CD3z-truncCAR(anti-CD19)

    Plasmid

    #215758

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR (CD247, CD19 Human)
    

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid

    #200679

    Purpose

    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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