161,273 results

1. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171379

   Purpose

   Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized Cas13X.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB591

   Plasmid

   #141096

   Purpose

   E. coli-Lactobacilli shuttle vector for recombineering template cloning

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-USP33

   Plasmid

   #22601

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP33 (USP33 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-FOXM1

   Plasmid

   #153136

   Purpose

   Expression of Flag tagged human FOXM1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXM1 (FOXM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-TO-TVMVP

   Plasmid

   #116078

   Purpose

   Expresses TVMV protease

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   TVMVP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIPZ-p21-HA(RxL)

   Plasmid

   #215111

   Purpose

   Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

   Promoter

   CMV (Tet inducible)

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUS_anion

   Plasmid

   #98656

   Purpose

   Expresses untagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. DsRed-rab11 WT

   Plasmid

   #12679

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Use

   Tags

   DsRed and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pThpR

    Plasmid

    #190613

    Purpose

    Expresses GFP in response to tetrahydropapaverine in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    THP4.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid

    #234953

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. TLCV2-Scal-T2A-EGFP-Puro

    Plasmid

    #234949

    Purpose

    Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TLCV2-ApaLI-T2A-EGFP-Puro

    Plasmid

    #234948

    Purpose

    Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Integrin-αv Headpiece M400GC

    Plasmid

    #159637

    Purpose

    Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    alpha v headpiece M400GC
    

    Use

    Tags

    6x His and Strep II

    Expression

    Mammalian

    Mutation

    M400C

    Promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDest-633

    Plasmid

    #161877

    Purpose

    Standard Gateway (attR1-attR2) vector for expression of His6-GST fusions using the Bac-to-Bac insect cell system

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Tags

    His6-GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-hPIKfyve

    Plasmid

    #121148

    Purpose

    Expresses GFP-tagged human PIKfyve in mammalian cells.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

    Insert

    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMYH9-WT-V5

    Plasmid

    #183512

    Purpose

    Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-FLAG-puro-PTPIP51(1-470)

    Plasmid

    #170536

    Purpose

    Expresses PTPIP51(1-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223_PTEN_WT

    Plasmid

    #81737

    Purpose

    Gateway Donor vector containing PTEN , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-FRA1

    Plasmid

    #188665

    Purpose

    Expresses murine FRA1 (Fosl1)

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP-N1 hMOG-beta1-EGFP

    Plasmid

    #160978

    Purpose

    Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

    Insert

    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMC1SG5

    Plasmid

    #31282

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 May 27. ():.

    Insert

    Cry1 (Cry1 Mouse)
    

    Use

    Tags

    Flag, His, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pGEMT-PT2A-GFP-Tdtomato-iRFP670

    Plasmid

    #111812

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    GFP
    Tdtomato
    iRFP670
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LeGO-EBFP2

    Plasmid

    #85213

    Purpose

    Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    EBFP2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-EGFP-Rac1(wt)

    Plasmid

    #13719

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    EGFP-Rac1(wt) (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWPI-GFP-3xFLAG

    Plasmid

    #201639

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. TFORF1485

    Plasmid

    #144176

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NANOG (NANOG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    David Root, John Doench

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223-CAMK2D

    Plasmid

    #23814

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

30. 3xnls-mNeonGreen

    Plasmid

    #98875

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    3xNLS
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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