163,445 results

1. pET21-pelB-LaG94-1--LaG94-14

   Plasmid

   #228973

   Purpose

   For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-1--LaG94-14
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-GST-1

   Plasmid

   #228983

   Purpose

   For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaTdT-1

   Plasmid

   #228974

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaRIgG-7

   Plasmid

   #228997

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaRIgG-1

   Plasmid

   #228995

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21-pelB-LaMIgG-5

   Plasmid

   #228991

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-5
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCit(P5f-P13f)3-gs

   Plasmid

   #228080

   Purpose

   Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   mCit, P5f, P13f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSCD-TVMVP

   Plasmid

   #226226

   Purpose

   Express TVMVP

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   TVMVP (TVMVgp1 Tobacco vein mottling virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PCR4-beta-globin::lacZ-H11

   Plasmid

   #227000

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)

   Depositor

   Guillaume Andrey

   Article

   Darbellay et al Nat Commun. 2024 Jun 6;15(1):4820. doi:

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-globin

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TagBFP(P6f)3(P14f)3-gs

    Plasmid

    #228081

    Purpose

    Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, P6f, P14f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid

    #218207

    Purpose

    Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

    Depositor

    Katarzyna Koltowska

    Article

    Panara et al Development. 2024 May 1;151(9):dev202525

    Insert

    -2.1prox1a_1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_hCAR.Variant3

    Plasmid

    #209133

    Purpose

    The ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    constitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223 MERS-CoV NSP6_stop

    Plasmid

    #168633

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    NSP6 (orf1ab )
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pDONR223 MERS-CoV NSP3_nostop

    Plasmid

    #168608

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    NSP3 (orf1ab )
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Kv1.2 K+ channel scFv [K14/16]

    Plasmid

    #182064

    Purpose

    Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRESN2_GFP_YTA_Champ1_328-354

    Plasmid

    #105639

    Purpose

    Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Use

    Tags

    GFP YTA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BASIC_SEVA_5a7_pKD46.10

    Plasmid

    #178937

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. CAG-EnRRbpj

    Plasmid

    #160925

    Purpose

    dominant-negative construct to inhibit Notch signaling

    Depositor

    Mark Emerson

    Article

    Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

    Insert

    EnRRbpj
    

    Use

    Tags

    Expression

    Mutation

    fused with an engrailed repressor

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Flag-mRIPK1-S321A

    Plasmid

    #115339

    Purpose

    Mammalian expression of Flag-mRIPK1-S321A

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321A

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAd5-B1

    Plasmid

    #122551

    Purpose

    Block 1 for AdenoBuilder genome assembly

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 1-3759
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFOXA2-T2A-mTagBFP2-PGK-Neo

    Plasmid

    #223191

    Purpose

    BFP reporter plasmid targeting the human FOXA2 gene

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
    

    Use

    Cre/Lox; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMSCV-OPA1

    Plasmid

    #26047

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF2538

    Plasmid

    #144036

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF3081

    Plasmid

    #144557

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

    Plasmid

    #177920

    Purpose

    Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-Squash-SAM-sensor 5-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCAGFP

    Plasmid

    #32748

    Depositor

    Jeanne Hardy

    Article

    Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

    Insert

    GFP
    

    Use

    Tags

    DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

    Expression

    Mammalian

    Mutation

    S65T

    Promoter

    CMV

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLARRYv2-EGFP

    Plasmid

    #233208

    Purpose

    Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge