We narrowed to 11,713 results for: NSI

1. PXPR007 sgATXN1L-2

   Plasmid

   #74963

   Purpose

   Cas9 + sgATXN1L-2 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ATXN1L (ATXN1L Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-TetO-hSOX17

   Plasmid

   #206389

   Purpose

   Expresses human SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-GRAB_CCK1.0

   Plasmid

   #208674

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Linked Free NES

   Plasmid

   #182485

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 shETV1-B

   Plasmid

   #74974

   Purpose

   shETV1 shRNA

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV1 (ETV1 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNUT N6His K206E/K534A hTFNG

   Plasmid

   #70133

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

   Insert

   N6His K206e/K534A hTFNG (TF Human)
   

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   changed Lys206 to a glutamic acid and Lys534 to a…

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-1574/+120

   Plasmid

   #35539

   Depositor

   Greg Goodall

   Article

   Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

   Insert

   promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Promoter

   none

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB_NPYmut

   Plasmid

   #208679

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

   Plasmid

   #149507

   Purpose

   Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Transient expression

   Tags

   TEV-3XHA-3XFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized Sequence, p.LL256-257AA

   Promoter

   CMV

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MKV937/nlp-1p::FlincG3

    Plasmid

    #140508

    Purpose

    This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

    Depositor

    Miri VanHoven

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    FlincG3
    

    Expression

    Worm

    Promoter

    nlp-1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_ARHGAP32-1/1

    Plasmid

    #91312

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP32-1/1 (ARHGAP32 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAG8Ha-SERBP1(149-400)

    Plasmid

    #176516

    Purpose

    Expresses the human C-terminal SERBP1 149-400 construct.

    Depositor

    David Libich

    Article

    Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

    Insert

    SERBP1 149-400 (SERBP1 Human)
    

    Tags

    His8 tag, linker, TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-148 and 401-408

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid

    #108484

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_ABL1-1/1

    Plasmid

    #91521

    Purpose

    Protein expression and purification of human SH3 domain construct ABL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ABL1-1/1 (ABL1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP-SFFV-mtagBFP-2A RIGI K270A

    Plasmid

    #167290

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    K270A

    Promoter

    SFFV

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3-hALDOA D34S

    Plasmid

    #210810

    Purpose

    To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 34 of ALDOA mutated to serine

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-SV40P-Tet2 enhancer H1

    Plasmid

    #63883

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet2

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet2 enhancer fragment H1
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCRII apoEb

    Plasmid

    #121901

    Purpose

    Plasmid for making zebrafish apoEb in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoeb (apoeb Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_ASAP2-1/1

    Plasmid

    #91504

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP2-1/1 (ASAP2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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