We narrowed to 12,339 results for: pcdna
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NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
TagsNanoLucExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF4A8
Plasmid#31114DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED19
Plasmid#15420DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pStreptagII 3C SMO
Plasmid#234995PurposeExpression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptorDepositorAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
opto-E-cad GFP
Plasmid#203327PurposeExpresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cellsDepositorInsertPhotoswitchable - E-cadherin with GFP tag (CDH1 Human)
TagsGFPExpressionMammalianMutationAddition of Aslov2 domain at T133 of human E-cadh…Available SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED20
Plasmid#15421DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pC1-dmito-oROS-HT
Plasmid#216418PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.DepositorInsertdmito-oROS-HT
ExpressionMammalianPromoterCMVAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM
Plasmid#164783PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
TagsGLucExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1-oROS-HT-CaaX
Plasmid#216417PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.DepositorInsertoROS-HT-CaaX
ExpressionMammalianPromoterCMVAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/20]
Plasmid#206744PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GANLS-DEVD-BNES
Plasmid#50835PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddGFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Klf4
Plasmid#172870PurposeMammalian expression vector with myc-tagged mouse Klf4 coding sequenceDepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-CyOFP1
Plasmid#105799PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-CyOFP1ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/44]
Plasmid#206743PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H74
Plasmid#170338PurposemTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-pTau nanobody (A2) with sortase tag
Plasmid#233218PurposeMammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.DepositorInsertAnti-pTau nanobody (A2)
TagsSortase tag, His tagExpressionMammalianAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
wt MPC1-FLAG
Plasmid#228903PurposeExpression of wild-type mouse MPC1 protein with a C terminal FLAG tagDepositorAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Grhl3
Plasmid#172869PurposeMammalian expression vector with myc-tagged mouse Grhl3 coding sequenceDepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/8]
Plasmid#199429PurposeMammalian expression of GFP scFv. Derived from hybridoma N86/8.DepositorInsertrecombinant mouse scFv targetingGFP (Aequorea victoria)
ExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRFP-PA-GFP-RhoAQ63L
Plasmid#187282PurposeExpress pmRFP-PAGFP-RhoA Q63LDepositorAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G nSaCas9
Plasmid#135365PurposeExpression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitutionDepositorInsertminiABEMax V82G nSaCas9
UseCRISPRExpressionMammalianMutation(V82G)Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaMK2rep
Plasmid#239622PurposeCaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.DepositorInsertSpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
TagsSpot, myc, and sGFP2ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
ExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-FHA1
Plasmid#87711PurposeEncodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).DepositorInsertDronpa-FHA1
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
TagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR149
Plasmid#106049PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)DepositorInsertOpto-GPR149 (GPR149 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQ54-VC
Plasmid#175060Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cellsDepositorInsertpolyQ54 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQ25-VC
Plasmid#175062Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cellsDepositorInsertpolyQ25 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only
