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pDONR223_H2AFX_p.K134N

Plasmid

#81275

Purpose

Gateway Donor vector containing H2AFX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

H2AFX (H2AX Human)

Use

Gateway Cloning

Mutation

K134N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-BirA

Plasmid

#71752

Purpose

Aurein1.2+36GFP-BirA fusion for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2+36GFP-BirA

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Tags

Knob

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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GEM

Plasmid

#55680

Purpose

expression of mouse GEM

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

GEM (Gem Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
REM2

Plasmid

#55682

Purpose

expression of mouse REM2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM2 (Rem2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xt-VSP

Plasmid

#51887

Purpose

expression of Xt-VSP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

see comments*

Promoter

CMV

Available Since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS9

Plasmid

#20248

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS9

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pBS cPtc2 (CT#4)

Plasmid

#13942

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 2001 Nov 1. 239(1):15-29.

Insert

Ptc2 (PTCH2 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pMIIS638

Plasmid

#238210

Purpose

Expresses native SpTasH TIGR locus. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

SpTasH

Expression

Bacterial

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEHAC1- HA-BirA-Traf6

Plasmid

#232585

Purpose

Express BirA tagged to the N-terminus of TRAF6.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA -TRAF6 (TRAF6 Human)

Tags

HA

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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enOgeuIscB with ωRNA-v2

Plasmid

#220939

Purpose

CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Inserts

CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBM-strep-UCP1

Plasmid

#216207

Purpose

for expression of human strep-tagged UCP1 protein in BacMam system

Depositor

Lei Chen

Article

Kang et al Nature. 2023 Aug;620(7972):226-231. doi:

Insert

UCP1 (UCP1 Human)

Tags

strep

Expression

Mammalian

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB

Plasmid

#216233

Purpose

S. aureus USA300 GyrB Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cas12c-sgRNA_BbsI_CAM

Plasmid

#183221

Purpose

Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-tetR-T2A-iRFP

Plasmid

#176028

Purpose

construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

tetR
iRFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pMOX

Plasmid

#160922

Purpose

Methanol oxidase (MOX) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Methanol oxidase promoter

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMTP112 (pMS26_miniTn6900(KanR))

Plasmid

#164260

Purpose

Delivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

miniTn6900 element

Use

Bacterial delivery vector for tn7 att site

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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