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pDONR223-ZNF219

Plasmid

#88118

Purpose

Donor Vector containing ZNF219 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF219 (ZNF219 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF8

Plasmid

#88528

Purpose

Donor Vector containing ZNF8 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF8 (ZNF8 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF175

Plasmid

#88551

Purpose

Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF175 (ZNF175 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF132

Plasmid

#88563

Purpose

Donor Vector containing ZNF132 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF132 (ZNF132 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF32

Plasmid

#88309

Purpose

Donor Vector containing ZNF32 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF32 (ZNF32 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1

Plasmid

#166846

Purpose

Expresses Atg1 with ATG1 3'UTR in yeasts.

Depositor

Daniel Klionsky

Publication

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLAG-SFPQY527A

Plasmid

#166961

Purpose

Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Mutation

SFPQ-Y527A

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPD107 VP16-ZF43(RARR)

Plasmid

#138812

Purpose

Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RARR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138739

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138747

Purpose

Integration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

CAG

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CP1041-ABE8e-dimer

Plasmid

#138499

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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DYNLL1

Plasmid

#132530

Purpose

Human LC8-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Publication

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL1 (DYNLL1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K6-2xCSTREP

Plasmid

#136155

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K6

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K6

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSB4C5_J23101-vdh

Plasmid

#128130

Purpose

Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Publication

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SUN-K08 vpr

Plasmid

#115832

Purpose

Encodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSUN Vpr (FR751162)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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wt Cus2p

Plasmid

#73046

Purpose

Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

Depositor

Aaron Hoskins

Publication

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Promoter

T7

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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ESR1-Tol2

Plasmid

#113382

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Tol2 reporter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only
rs214-minor

Plasmid

#113380

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rs2149943 minor allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB1omega2_SF (GB0466)

Plasmid

#68243

Purpose

Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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