165,307 results

1. Ornithine transcarbamylase (OTC)

   Plasmid

   #71877

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC (Otc Rat)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSYN1-fDIO-tTA

   Plasmid

   #166597

   Purpose

   Encodes Flp-dependent tTA

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   tTA
   

   Use

   AAV

   Promoter

   human synapsin 1

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-FLAG-Luciferase

   Plasmid

   #58792

   Purpose

   Express FLAG-tagged Luciferase in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   firefly Luciferase
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-C-mScarlet-EFS-Blast

   Plasmid

   #227260

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMax_ScaI_BFP

   Plasmid

   #177824

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP
   

   Expression

   Mammalian

   Mutation

   5' inserted ScaI RE cut site

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Syn-DIO-NaChBac-dTomato

   Plasmid

   #236252

   Purpose

   Cre dependent NaChBac-TdTomato expression

   Depositor

   Janelia Viral Tools

   Article

   Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

   Insert

   NaChBac-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.2 V5-DEST 3XFlag-SRSF3

   Plasmid

   #46736

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   SRSF3 (SRSF3 Human)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-ClopHensor

   Plasmid

   #25938

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   ClopHensor
   

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Available Since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS307

    Plasmid

    #51785

    Purpose

    an integrating (YIp) vector with a LYS2 selectable marker

    Depositor

    David Stillman

    Article

    Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N1-TFE3

    Plasmid

    #38120

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFE3 (TFE3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Tags

    mGFP5, GUS

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHM129

    Plasmid

    #225534

    Purpose

    Encodes destablized mCherry under AR8 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    mCherry
    BFP
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP hsp70

    Plasmid

    #15215

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 (HSPA1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH_Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65258

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205317

    Purpose

    All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISYmu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Tags

    his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge