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pFA6a-link-yomApple-Kan

Plasmid

#44957

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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LI Intron c1

Plasmid

#54433

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Intron element for construction of silencing vectors (intron1 of AtWRKY33)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-SpHis5

Plasmid

#44842

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pRset-OppA

Plasmid

#207869

Purpose

Bacterial expression of poly-his-tagged L. lactic OppA

Depositor

Mathew Tantama

Article

Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

Insert

OppA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102G

Plasmid

#112176

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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EB3-mTurquoise2

Plasmid

#98825

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

EB3

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PEP104E

Plasmid

#97390

Purpose

binary construct of mitochondria-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Use

Tags

mitochondria target

Expression

Plant

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pAJM.717

Plasmid

#108517

Purpose

PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTet*-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-UGAcS

Plasmid

#172137

Purpose

bacteria expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Use

Tags

Expression

Bacterial

Mutation

H605Q- please see depositor comments

Promoter

araBAD

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CIp10 - YemVenus (OPT High)

Plasmid

#163122

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

YemVenus OPT High

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJYDNgp

Plasmid

#162453

Purpose

Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-2Glinker-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

HA nad myc and eUnaG2 fluorescent protein

Expression

Mutation

Promoter

GAL1

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1-moxVenus

Plasmid

#103993

Purpose

Plasmid encoding moxVenus

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxVenus

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pZH516

Plasmid

#102669

Purpose

Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomEos2-Kan

Plasmid

#44944

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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