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We narrowed to 63,685 results for: cloning

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Showing: 7701 - 7720 of 63685 results

  1. pcDXC3MS

    Plasmid
    #49030
    Purpose
    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide
    Depositor
    Raimund Dutzler, Eric Geertsma
    Publication
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Tags
    3C protease site (PreScission site), MYC, and str…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49030-plasmid-map-sequence-id-71988

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  3. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    219819-plasmid-map-sequence-id-440390

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  5. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Publication
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Expression
    Yeast
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

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  7. R777-E243 Hs.RASGRP1

    Plasmid
    #70527
    Purpose
    Gateway ORF clone of human RASGRP1 [NM_005739.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASGRP1 (RASGRP1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70527-plasmid-map-sequence-id-125976

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  8. R777-E083 Hs.FGFR1

    Plasmid
    #70367
    Purpose
    Gateway ORF clone of human FGFR1 [NM_001174063.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR1 (FGFR1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70367-plasmid-map-sequence-id-125816

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  9. R777-E035 Hs.DUSP2

    Plasmid
    #70319
    Purpose
    Gateway ORF clone of human DUSP2 [NM_004418.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP2 (DUSP2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70319-plasmid-map-sequence-id-125768

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  11. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Publication
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  18. pVP077

    Plasmid
    #222029
    Purpose
    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Publication
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    All Esp3i sites domesticated
    Available since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222029-plasmid-map-sequence-id-447312

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Showing: 7701 - 7720 of 63685 results