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pFH59

Plasmid

#128185

Purpose

Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH81

Plasmid

#128192

Purpose

Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ScCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL14

Plasmid

#51794

Purpose

Inducible expression of TAL14 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL14

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF2YE

Plasmid

#107872

Purpose

Bacterial expression of FGF2phosphomimic-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-phosphomimic

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

Y82E

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pANY2

Plasmid

#112196

Purpose

This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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AP-SEMA3C (chick)

Plasmid

#29449

Depositor

Jonathan Raper

Article

Feiner et al Neuron. 1997 Sep . 19(3):539-45.

Insert

AP-SEMA3C (SEMA3C Chicken)

Tags

AP

Expression

Mammalian

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pCRISPaint-myc-PuroR

Plasmid

#80961

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

myc tag

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBS cGli3 (CT#34)

Plasmid

#13953

Depositor

Cliff Tabin

Article

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli3 (GLI3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
LI C-D destab GFP c1

Plasmid

#54454

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

destablized GFP

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18 (1wa3)

Plasmid

#80123

Purpose

PH-4GS-I3-01-myc-Late1 (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-25 (1wa3)

Plasmid

#80124

Purpose

I3-01p-myc-Late1-PH (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-25 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01(∆L1+∆L2)-His

Plasmid

#84511

Purpose

E.coli version of EPN01(∆L1+∆L2)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01(∆L1+∆L2)-His

Tags

His tag

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HMEKc-srcM

Plasmid

#1107

Depositor

Susan Lindquist

Article

Xu et al Proc Natl Acad Sci U S A 1999 Jan 5;96(1

Insert

c-src

Expression

Yeast

Mutation

MET3 promoter inserted in EcoRI and BamHI of pRS3…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pICH45173

Plasmid

#50259

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS1B (AT5g38430, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1479

Plasmid

#50291

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH78141

Plasmid

#50293

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (Oryza sativa)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM5331

Plasmid

#50294

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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