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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-NMNAT2

Plasmid

#133260

Purpose

Express FLAG-tagged NMNAT2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT2 (NMNAT2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-VP1SA11

Plasmid

#89162

Purpose

Express Rotavirus SA11 VP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP1

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 PuroR

Plasmid

#84386

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

Depositor

Ghassan Mouneimne

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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mScarletI3-6DG5-MAPPER

Plasmid

#222421

Purpose

To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

MAPPER

Tags

mScarletI3-6DG5

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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MBD-IFP2.0C

Plasmid

#215748

Purpose

Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-KDEL

Plasmid

#124316

Purpose

Expresses ER specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

KDEL Seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTCF120 H-2D(b)

Plasmid

#180454

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2D(b)

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mCasp1

Plasmid

#208781

Purpose

Inducibly expresses mouse caspase1 fused to light activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mCaspase1 Cry2 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry, Cry2

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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