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pDONR223_SPATA8_p.E18K

Plasmid

#81272

Purpose

Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPATA8 (SPATA8 Human)

Use

Gateway entry vector

Mutation

E18K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB42_p.A61T

Plasmid

#81273

Purpose

Gateway Donor vector containing RAB42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAB42 (RAB42 Human)

Use

Gateway entry vector

Mutation

A61T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPATA8_p.P83T

Plasmid

#81260

Purpose

Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPATA8 (SPATA8 Human)

Use

Gateway entry vector

Mutation

P83T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4A

Plasmid

#224448

Purpose

Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYNSL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TERT - WT

Plasmid

#213827

Purpose

Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

human TERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2A

Plasmid

#224440

Purpose

Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQTTL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLIRP_WT

Plasmid

#82288

Purpose

Gateway Donor vector containing SLIRP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLIRP (SLIRP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN-MEF2C

Plasmid

#232627

Purpose

Lentiviral plasmid expressing mouse MEF2C.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

MEF2C (Mef2c Mouse)

Use

Lentiviral

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ATF4

Plasmid

#192911

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ATF4 (ATF4 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-i53

Plasmid

#92170

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Promoter

CMV

Available Since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_WT

Plasmid

#81856

Purpose

Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TERT - R865C

Plasmid

#213926

Purpose

Expresses Mutant TERT R865C

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Mutant TERT R865C (TERT Human)

Use

Lentiviral

Tags

6x His Tag

Expression

Mammalian

Mutation

Arginine 865 changed to Cysteine

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FOXL2

Plasmid

#192914

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 (FOXL2 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_WT

Plasmid

#82216

Purpose

Gateway Donor vector containing SMAD4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Neo

Plasmid

#99378

Purpose

Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

hNGN2-P2A-Neo (NEUROG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBL1XR1_WT

Plasmid

#82059

Purpose

Gateway Donor vector containing TBL1XR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBL1XR1 (TBL1XR1 Human)

Use

Gateway entry vector

Mutation

L512P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT6_WT

Plasmid

#81950

Purpose

Gateway Donor vector containing STAT6, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT6 (STAT6 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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