159,117 results

1. pMT006

   Plasmid

   #64761

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYS16

   Plasmid

   #202082

   Purpose

   Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   CT mNG-T2A-PuroR Minicircle Parent
   

   Use

   CRISPR

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAX APP 695

   Plasmid

   #30137

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type splice variant 695

   Promoter

   Available since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLIK 3XFLAG-wtGATA6-3XAU1 neo

   Plasmid

   #72618

   Purpose

   Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Lentiviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Tight

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV4Neo-murine IL-17RA-HA

   Plasmid

   #46859

   Purpose

   expresses mouse IL-17RA

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA (Il17ra Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

   Plasmid

   #117168

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TEVSH

   Plasmid

   #125194

   Purpose

   His-tagged TEV protease with solubility increasing mutations

   Depositor

   Helena Berglund

   Article

   van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

   Insert

   TEV_SH
   

   Use

   Tags

   V5 His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG26

   Plasmid

   #35127

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   hph
   

   Use

   Yeast replicating

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dSpyCas9-p300-P2A-CASANOVA

   Plasmid

   #113129

   Purpose

   Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   dCas9-p300-P2A-CASANOVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcU6_3 sgRNA

    Plasmid

    #92359

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chick U6.3

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge