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pHAGE-PTEN-R173C

Plasmid

#116632

Purpose

Lentiviral expression of PTEN R173C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R173C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-D24G

Plasmid

#116619

Purpose

Lentiviral expression of PTEN D24G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

D24G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA-IFITM3-F8-TAG-C71A

Plasmid

#122476

Purpose

expresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

Depositor

Howard Hang

Article

Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

Insert

IFITM3 (IFITM3 Human)

Tags

HA

Expression

Mammalian

Mutation

Changed Phenylalanine 8 to amber codon, cysteine …

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 3xRA

Plasmid

#121115

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A, R20A, R22A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Mutation

R19A, R20A, R22A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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ESR1-Tol2

Plasmid

#113382

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Tol2 reporter

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only
KAT6B-luciferase

Plasmid

#113353

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

KAT6B-enhancer (KAT6B Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM28521

Plasmid

#105359

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

EC1.2en-EC1.1p fusion promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-ndufs2 guide

Plasmid

#107601

Purpose

An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

ndufs2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_3

Plasmid

#73535

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pRSETB-roGFP1-R10

Plasmid

#83310

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R10

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/F223R/S202K/K41D

Promoter

T7

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUP95PDZGW (C6)

Plasmid

#74022

Purpose

lentiviral expression of Psd95 shRNA and Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

PDZ

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya-PDHA1-N-10

Plasmid

#56349

Purpose

Localization: Mitochondria, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

PDHA1 (PDHA1 Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-PDHA1-N-10

Plasmid

#56356

Purpose

Localization: Mitochondria, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

PDHA1 (PDHA1 Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Calnexin-N-14

Plasmid

#56350

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Calnexin (CANX Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya1-CENPB-N-22

Plasmid

#56351

Purpose

Localization: Nucleus/Centromeres, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

CENPB (CENPB Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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TAL3515

Plasmid

#47234

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pls1-right (pls1 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3514

Plasmid

#47233

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pls1-left (pls1 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3523

Plasmid

#47242

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-rbfox2-right (rbfox2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3522

Plasmid

#47241

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-rbfox2-left (rbfox2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only