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pBTK1050

Plasmid

#209540

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Publication

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available since

Feb. 9, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LentiCRISPRCreb5gRNA

Plasmid

#195021

Purpose

Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Creb5 gRNA (CREB5 Bovine)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF1

Plasmid

#200375

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGA-red-maxi

Plasmid

#196337

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-4

Plasmid

#197694

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

CmYLCV promoter

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#197699

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

CmYLCV promoter

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-Z-SpCas9-gRNA-repeat-polyT-11

Plasmid

#197781

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

gRNA repeat for U6 sgRNA assembly

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0202

Plasmid

#185626

Purpose

MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF532

Plasmid

#88740

Purpose

Donor Vector containing ZNF532 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF532 (ZNF532 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF688_isoform1

Plasmid

#88779

Purpose

Donor Vector containing ZNF688 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF688 (ZNF688 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF846

Plasmid

#88355

Purpose

Donor Vector containing ZNF846 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF846 (ZNF846 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0818

Plasmid

#177020

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase; geranyl diphosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF800

Plasmid

#88573

Purpose

Donor Vector containing ZNF800 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF800 (ZNF800 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF804B

Plasmid

#88756

Purpose

Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF804B (ZNF804B Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A-SRDX

Plasmid

#129684

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b-SRDX

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_L3(GSSSSGGSSSG)

Plasmid

#131210

Purpose

Cloning vector containing A-B-C casette with linker L3 in site B

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSSGGSSSG llinker

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L2(GSSGGSSG)

Plasmid

#131209

Purpose

Cloning vector containing A-B-C casette with linker L2 in site B

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSGGSSG linker

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L1(GSSSG)

Plasmid

#131208

Purpose

Cloning vector containing A-B-C casette with linker L1 in site B

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSG linker

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 dCD

Plasmid

#121117

Purpose

conditional expression

Depositor

Emily Dykhuizen

Publication

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 72-389 (CBX8 Human)

Use

Lentiviral

Tags

V5

Mutation

contains amino acids 77-389

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCK032

Plasmid

#105412

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain (glucocorticoid receptor)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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