We narrowed to 8,677 results for: sgRNA

1. pUDR217

   Plasmid

   #113872

   Purpose

   expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

   Depositor

   Jack Pronk, Jean-Marc Daran

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA9-MPH2-3 sgRNA10-MAL11
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pURD214

   Plasmid

   #113871

   Purpose

   expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

   Depositor

   Jack Pronk, Jean-Marc Daran

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA5 HXT13-15-16 sgRNA2-HXT2
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUDR220

   Plasmid

   #113873

   Purpose

   expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

   Depositor

   Jack Pronk, Jean-Marc Daran

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA8-HXT10 sgRNA7-HXT9-11-12
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDR295

   Plasmid

   #113874

   Purpose

   expression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectively

   Depositor

   Jack Pronk, Jean-Marc Daran

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv4

   Plasmid

   #221432

   Purpose

   Lentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(RUNX1T1 g3)-PGKpuroBFP-W

   Plasmid

   #211984

   Purpose

   Express gRNA against RUNX1T1 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting RUNX1T1 (RUNX1T1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(RUNX1T1 g7-5)-PGKpuroBFP-W

   Plasmid

   #211985

   Purpose

   Express gRNA against RUNX1T1 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting RUNX1T1 (RUNX1T1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCKOv3

   Plasmid

   #209046

   Purpose

   Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_051_dCas9-KRAB-HA

    Plasmid

    #228936

    Purpose

    All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cbh_v5 AAV-CBE C-terminal

    Plasmid

    #137176

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cbh_v5 AAV-ABE C-terminal

    Plasmid

    #137178

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid

    #178209

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.HA and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pABE8e-NG-403Q-Nterm-AAV

    Plasmid

    #194651

    Purpose

    N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG N-term
    

    Use

    AAV

    Tags

    BPNLS and NpuN spilt intein

    Promoter

    TNNT2

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pABE8e-NG-403Q-Cterm-AAV

    Plasmid

    #194652

    Purpose

    C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG C-term
    

    Use

    AAV

    Tags

    BPNLS and NpuC split intein

    Promoter

    TNNT2

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCKO

    Plasmid

    #73311

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-BLAST flag-CAD

    Plasmid

    #169818

    Purpose

    Expresses C-terminal flag-tagged CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCCC -> AGTC silent mutations at nt527-530 eli…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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