We narrowed to 8,781 results for: construct

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pRB144

Plasmid

#180175

Purpose

pCDNA3.1 Construct GluN1 A714L

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-386)

Plasmid

#61936

Purpose

Expression of hRPN13 (285-386) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-386) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, stop codon intro…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_FUT8-1/1

Plasmid

#91270

Purpose

Protein expression and purification of human SH3 domain construct FUT8-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FUT8-1/1 (FUT8 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF6-1/1

Plasmid

#91515

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF6-1/1 (ARHGEF6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

Plasmid

#186745

Purpose

dCas9-10XGcn4-P2A-mCherry expression construct

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

Cas9-10XGcn4-P2A-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

Plasmid

#179429

Purpose

Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-5kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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DNMT1 KO Neo

Plasmid

#16649

Depositor

Bert Vogelstein

Article

Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

Insert

DNMT1 knock-out construct (DNMT1 Human)

Expression

Bacterial

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pET29-hMIGA2(306-593)-6xhis

Plasmid

#192837

Purpose

Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

6xhis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ECON

Plasmid

#82553

Purpose

Epigenetic effector construct for targeted methylation

Depositor

Joseph Irudayaraj

Article

Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)

Tags

HALO and mCherry

Expression

Mammalian

Mutation

N-terminal photolyase homology region domain of C…

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hNCL

Plasmid

#103805

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK2-1/1

Plasmid

#91482

Purpose

Protein expression and purification of human SH3 domain construct DOCK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK2-1/1 (DOCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP1106

Plasmid

#119249

Purpose

construction intermediate

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF16-1/1

Plasmid

#91334

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF16-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF16-1/1 (ARHGEF16 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF26-1/1

Plasmid

#91342

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF26-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF26-1/1 (ARHGEF26 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-2/5

Plasmid

#91385

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-2/5 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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L309-myc-seipin-ΔCT

Plasmid

#64062

Purpose

myc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

Insert

bscl2 (Bscl2 Mouse)

Use

Lentiviral

Tags

myc epitope

Expression

Mammalian

Mutation

Deletion of C-terminal Asp338–Ser443

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pDestTol2-pax3a-kcnj13-IRES-EGFP

Plasmid

#164950

Purpose

Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

pax3a promoter -5.4k

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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