We narrowed to 51,304 results for: des.1
-
Plasmid#222659PurposeMammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-termDepositorInsertZc3h12c 1-244 aa (Zc3h12c Mouse)
TagsFLAGExpressionMammalianMutationmutated asparagine 245 to a stop codonPromoterCMVAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-CD28 CAR hinge (I114 – P152)
Plasmid#224929PurposeExpression GST fusion CD28 CAR hinge (I114-P152) in Escherichia coli.DepositorInsertCD28 CAR hinge (I114 – P152) (TMIGD2 Human)
Tagsglutathione-S-transferaseExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
TagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A pMT2
Plasmid#206110Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin bindingDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-ZNF91-BFP
Plasmid#202823PurposeLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene
UseLentiviralExpressionMammalianPromoterU6 - twoAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M
Plasmid#146950PurposeInsect Expression of DmAGO1-del942-971-GGSGDepositorInsertDmAGO1-del942-971-GGSG (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M
Plasmid#146951PurposeInsect Expression of DmAGO1-del942-971-GSGDepositorInsertDmAGO1-del942-971-GSG (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M
Plasmid#146948PurposeInsect Expression of DmAGO1-del835-886-GGSGDepositorInsertDmAGO1-del835-886-GGSG (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M
Plasmid#146949PurposeInsect Expression of DmAGO1-del835-886-GSGDepositorInsertDmAGO1-del835-886-GSG (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations V315A and H…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Dcst1-3xHA
Plasmid#183540PurposeExpress Dcst1-3xHA under the mouse Clgn promoter.DepositorAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Slc1a3-CreERT2 Targeting Vector
Plasmid#129409PurposeGene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codonDepositorInsertSlc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335 Slc1a3 nickase 2
Plasmid#129411PurposeCas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 geneDepositorAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX459_GRHL1-exon5
Plasmid#177763PurposesgRNA for CRISPR/Cas9-mediated deletion of human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
UseCRISPRAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX459_GRHL1-exon8
Plasmid#177764PurposesgRNA for CRISPR/Cas9-mediated deletion of human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
UseCRISPRAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR13
Plasmid#69155Purposeread-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr IIDepositorInsertsUseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0, codon-optimzed index 1.…Promoterrps-27 and swsn-1Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHes1(2.5k)-luc
Plasmid#43806DepositorInsertHes1 Promoter (Hes1 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains the murine Hes1 promoterPromoterHes1 PromoterAvailable SinceMarch 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-Twin-Strep
Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcNucC
Plasmid#214050PurposeBacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorInsertEcNucC
ExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHes1(467 RBPj(-))-luc
Plasmid#43805DepositorInsertHes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains the murine Hes1 promoter (-467 to +46), …PromoterHes1 promoter fragment (-467 to +46) (RBPj-)Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
ExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only