We narrowed to 12,964 results for: BASE

Showing: 1521 - 1560 of 12964 results

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pCMV-dCpf1-BE-YE

Plasmid

#107686

Purpose

Expresses dLbCpf1-BE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE-YE

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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GA-DEVD-GB-NES

Plasmid

#61020

Purpose

green ddFP based caspase-3 biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddGFP B

Tags

HindIII and NES sequence LALKLAGLDIGS placed afte…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBUN501

Plasmid

#50582

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

zCas9D10A
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Mutation

Cas9D10A nickase, was derived from the zCas9 and …

Promoter

AtU6-26p and Ubi1p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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SPACE-ΔUGI (pRZ1836)

Plasmid

#140243

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE-ΔUGI

Expression

Mammalian

Mutation

V82G in TadA*, D10A in Cas9, R187W in pmCDA1

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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eA3A-BE3 (pRZ206)

Plasmid

#131315

Purpose

CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4RA-P2A-Puro

Plasmid

#112672

Purpose

Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4RA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-359

Plasmid

#131131

Purpose

gRNA expression vector for A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

A-to-G reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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hA3A-BE3 (pRZ215)

Plasmid

#131314

Purpose

CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3-PGK-Puro

Plasmid

#110845

Purpose

Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

TRE3G

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pWPT-/mEGFP-1T-IRES-mCherry

Plasmid

#190190

Purpose

Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Mutation

changed EGFP Kozak sequence inserting a C>T mo…

Promoter

EIF1-short

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CGBE1-VRQR (pBM1192)

Plasmid

#140254

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_VRQR-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y132D

Plasmid

#113425

Purpose

Expresses hA3A-BE3-Y132D in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y132D

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

Plasmid

#123611

Purpose

CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

nCas9-UGI-NLS-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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SECURE BE4max(R33A) (pBM608)

Plasmid

#140251

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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hAID-BE3 (pRZ352)

Plasmid

#131316

Purpose

CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-mA1-BE3

Plasmid

#113421

Purpose

Expresses mA1-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mA1-BE3 (Apobec1 Mouse, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-P2A-Puro

Plasmid

#110839

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mAID-BE3

Plasmid

#113420

Purpose

Expresses mAID-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mAID-BE3 (Aicda Mouse, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 FS10

Plasmid

#113956

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution…

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-PGK-Puro

Plasmid

#110843

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-miR-125b-1

Plasmid

#58990

Purpose

MSCV based expression vector - co-expresses mCherry and human miR-125b-1

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Inserts

mCherry fluorescent protein
miR-125b-1 (MIR125B1 Human)

Use

Retroviral; Mscv-based retroviral system

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hA1-BE3

Plasmid

#113418

Purpose

Expresses hA1-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA1-BE3 (APOBEC1 S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PB-BE3-2NLS

Plasmid

#119031

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-saBE3.9max_KKH C-terminal

Plasmid

#137181

Purpose

Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-saBE3.9max_KKH_C-terminal

Expression

Mammalian

Mutation

E782K;N968K;R1015H conferring recognition of NNNR…

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CS-154

Plasmid

#166230

Purpose

lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

Depositor

Nozomu Yachie

Article

Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

Inserts

U6-gRNA EGFP targetting
nCas9-PmCDA1-UGI-2A-mCherry

Use

CRISPR and Lentiviral

Promoter

CMV promoter and Human U6

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Gb2-K

Plasmid

#25766

Purpose

Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-cAICDA-BE3

Plasmid

#113422

Purpose

Expresses cAICDA-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE2

Plasmid

#120396

Purpose

Expresses HF2-BE2 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE2

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-1058

Plasmid

#135347

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058-UGI

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-BE3-PGK-Puro

Plasmid

#110842

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLL6 sgRNA(MS2)-MS2-hA3a D131E

Plasmid

#112132

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131E

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131E mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131E mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TmiR_Gb2-K

Plasmid

#25764

Purpose

Entry vector with TRE promoter driving mouse G beta 2 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3A-BE3-C101S

Plasmid

#113426

Purpose

Expresses hA3A-BE3-C101S in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-C101S (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_C101S

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3A-BE3-C106S

Plasmid

#113427

Purpose

Expresses hA3A-BE3-C106S in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-C106S (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_C106S

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-BE3-P2A-Puro

Plasmid

#110838

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 12,964 results for: BASE