-
Plasmid#117376PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR UBE2G2-202
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-RGS3-201
Plasmid#117359PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR RGS3-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-PLEKHG5-212
Plasmid#117355PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR PLEKHG5-212
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-CAMK2B-209
Plasmid#117342PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR CAMK2B-209
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-ARHGAP4-203
Plasmid#117338PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR ARHGAP4-203
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-SEMA5B-202
Plasmid#117362PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR SEMA5B-202
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-SEMA5B-211
Plasmid#117363PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR SEMA5B-211
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-RPLP1-201
Plasmid#117361PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR RPLP1-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-ASIC1
Plasmid#117339PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR ASIC1
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-ARF1-214
Plasmid#117337PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR ARF1-214
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-LEMD2-201
Plasmid#117349PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR LEMD2-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-PPM1F-201
Plasmid#117334PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR PPM1F-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-GLIS2-201
Plasmid#117331PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR GLIS2-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-CERS2-201
Plasmid#117330PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR CERS2-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT2
Plasmid#113093PurposeLuciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationSecond predicted miR-199a-3p binding site mutated…PromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT-both
Plasmid#113094PurposeLuciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth the predicted miR-199a-3p binding site muta…PromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF12
Plasmid#105593Purposeretrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertfull length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationsilent mutations to make it resistant to the targ…PromoterMSCV-LTRAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-puro-N3FLAG-MYB-del(TAD)
Plasmid#105597Purposeretrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistanceDepositorInsertMYB without TAD domain (Myb Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationdeletion of TAD (AA 275-327)PromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTS1037
Plasmid#169616PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1035
Plasmid#169614PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1036
Plasmid#169615PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1041
Plasmid#169619PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1040
Plasmid#169618PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH617
Plasmid#169606PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4 / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH615
Plasmid#169604PurposeTier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4 / PPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH616
Plasmid#169605PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4 / PPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH614
Plasmid#169603PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4 / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1043
Plasmid#169621PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1042
Plasmid#169620PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2326
Plasmid#169628PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2321
Plasmid#169626PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2329
Plasmid#169629PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2322
Plasmid#169627PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2320
Plasmid#169625PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2312
Plasmid#169622PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2317
Plasmid#169624PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSR1057
Plasmid#208742PurposeCharacterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)DepositorInsertPPgk
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPPgkAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2362_Tier3(SB)-rtTA-PuroR
Plasmid#169658PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W+P278A
Plasmid#183115PurposeExpresses flag-tagged p53 with both R248W and P278A mutations in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine and arginine 248 t…PromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
155_KRAS in pmiRGlo
Plasmid#78132Purposeluciferase reporter for miRNA activityDepositorInsertKRAS (KRAS Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB2C
Plasmid#195285PurposeBicistronic HSV-TK and Hygromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Hygro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#194703PurposeInducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsAPOBEC1-HA-P2A-mRubyExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2B
Plasmid#195284PurposeBicistronic HSV-TK and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Puro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W
Plasmid#183114PurposeExpresses flag-tagged p53 with R248W mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged arginine 248 to tryptophanPromoterEF1aAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only