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Showing: 1901 - 1950 of 4184 results

  9. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

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  10. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

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  11. pGFP(C3)-Vinculin

    Plasmid
    #30312
    Depositor
    Klaus Hahn
    Insert
    Vinculin (Vcl Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    F633S, V828E, L936P in mouse viniculin (NM_009502…
    Promoter
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    30312-plasmid-map-sequence-id-116004

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  12. KE-005

    Plasmid
    #133440
    Purpose
    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4A1 (EIF4A1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133440-plasmid-map-sequence-id-267708

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  14. 9AVA

    Plasmid
    #220884
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TREX1 (TREX1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220884-plasmid-map-sequence-id-436793

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  15. KE-009

    Plasmid
    #133441
    Purpose
    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4H (EIF4H Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133441-plasmid-map-sequence-id-267720

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  16. RG-001

    Plasmid
    #133439
    Purpose
    Heat 3 domain of human elF4G in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4G1 (EIF4G1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133439-plasmid-map-sequence-id-269573

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  17. 8VL7

    Plasmid
    #220883
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TREX1 (TREX1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220883-plasmid-map-sequence-id-436777

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  28. pBW016

    Plasmid
    #131820
    Purpose
    pET28b derrivative vector with T7-driven IPTG inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131820-plasmid-map-sequence-id-259076

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  37. pH6-2xmNeonC

    Plasmid
    #178463
    Purpose
    A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.
    Depositor
    Siegfried Musser
    Article
    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:
    Insert
    mNeonGreen dimer
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Free cysteine at the C-terminus of the dimer
    Promoter
    T7
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    178463-plasmid-map-sequence-id-392808

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  38. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Use
    Tags
    E6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Use
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

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  41. pSNRW-Z3-Nluc

    Plasmid
    #174486
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z3-Nluc
    Use
    Tags
    6x Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174486-plasmid-map-sequence-id-345289

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  43. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Use
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  44. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Use
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  46. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1901 - 1950 of 4184 results