We narrowed to 31,420 results for: Eng
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Plasmid#82383PurposesgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas14
Plasmid#82382PurposesgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas13
Plasmid#82381PurposesgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMY01AB-PRO
Plasmid#127714PurposeIntegrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEMY01CD-TER
Plasmid#127751PurposeIntegrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase
Plasmid#87741PurposeIPTG-inducible expression of T4 DNA ligase for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHisExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPExpressionMutationPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RLuc8
Plasmid#87121PurposeMammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.DepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationA55T, C124A, S130A, K136R, A143M, M185V, M253L, a…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-tag
Plasmid#55180PurposeFLAG-tagDepositorInsertFLAG
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-DsbC
Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-tag
Plasmid#55182PurposeHA-tagDepositorInsertHA tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-3
Plasmid#233295PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionMutationPromoterp14GAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-MsCHI
Plasmid#73394PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only