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Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_A34G_A35G
Plasmid#81232PurposeU6 telestem mutant (A34G/A35G) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (A34G/A35G)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_G96C_A97C
Plasmid#81235PurposeU6 telestem mutant (G96C/A97C) for in vivo studiesDepositorInsertU6 RNA with G96C/A97C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (G96C/A97C)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_U100C_U101C
Plasmid#81236PurposeU6 telestem mutant (U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with U100C_U101C mutation (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6 telestem mutant (G96C/A97C)PromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPhygSaCas9
Plasmid#123262PurposeExpresses Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorInsertStaphylococcus aureus Cas9 (NEWENTRY )
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPneoSagRNAH
Plasmid#123265PurposeExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorTypeEmpty backboneUseCRISPR; Leishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNeoSagRNAH
Plasmid#123266PurposeExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorTypeEmpty backboneUseCRISPR; Leishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0114
Plasmid#117553PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamianaDepositorInsertPromoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana
UseTagsExpressionPlantMutationPromoterAt U6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0029
Plasmid#117541PurposeLevel1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (LbCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0028
Plasmid#117540PurposeLevel1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (AsCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0014
Plasmid#117524PurposeLevel0 Golden Gate part: CDS, SpCas9-KADepositorInsertSpCas9-KA (with stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0009
Plasmid#117522PurposeLevel0 Golden Gate part: CDS, SpCas9-p (no stop codon)DepositorInsertSpCas9-p (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL90005
Plasmid#117520PurposeLevel0 Golden Gate part: CDS, SpCas9-h (no stop codon)DepositorInsertSpCas9-h (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0008
Plasmid#117535PurposeLevel0 Golden Gate part: CDS, AsCas12aDepositorInsertAsCas12a (with stop codon)
UseCRISPRTags3xHA and NLSExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818R
Plasmid#111956PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K818R mutationDepositorInsertHsh155 K818R
UseTagsExpressionBacterial and YeastMutationK818RPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 V783L
Plasmid#111955PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783L mutationDepositorInsertHsh155 V783L
UseTagsExpressionBacterial and YeastMutationV783LPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N747D
Plasmid#111954PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747D mutationDepositorInsertHsh155 N747D
UseTagsExpressionBacterial and YeastMutationN747DPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 3xFLAG
Plasmid#111960PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus.DepositorInsertHsh155 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Y826A
Plasmid#111952PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Y826A mutationDepositorInsertHsh155 Y826A
UseTagsExpressionBacterial and YeastMutationY826APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 V783A
Plasmid#111950PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783A mutationDepositorInsertHsh155 V783A
UseTagsExpressionBacterial and YeastMutationV783APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only