We narrowed to 5,554 results for: donor

1. ORFtag_TetR-SD_Frame1

   Plasmid

   #220981

   Purpose

   ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

   Depositor

   Alexander Stark

   Insert

   TetR repressor
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   PGK promoter

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScarlessHD-sfGFP-DsRed

   Plasmid

   #80811

   Purpose

   Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Kate O'Connor-Giles

   Insert

   sfGFP-piggyBac-DsRed
   

   Use

   Unspecified

   Tags

   sfGFP

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

   Plasmid

   #175572

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_gRNA_S.pyogenes_scaffold_BB

   Plasmid

   #226429

   Purpose

   PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

   Depositor

   Alon Goren

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScarlessHD-3xFLAG-DsRed

   Plasmid

   #80820

   Purpose

   Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Kate O'Connor-Giles

   Insert

   3xFLAG-piggyBac-DsRed
   

   Use

   Unspecified

   Tags

   3xFLAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScarlessHD-2xHA-DsRed

   Plasmid

   #80822

   Purpose

   Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Kate O'Connor-Giles

   Insert

   2xHA-piggyBac-DsRed
   

   Use

   Unspecified

   Tags

   2xHA

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD362

   Plasmid

   #137023

   Purpose

   SapTrap HaloTag-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized Halo Tag
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized Halo Tag

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-f1(+)-ACTB-mEGFP

   Plasmid

   #115909

   Purpose

   donor to insert EGFP at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-mEGFP (ACTB Human)
   

   Use

   Donor template

   Tags

   EGFP

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-f1(+)-ACTB-iTagRFP-T

    Plasmid

    #115915

    Purpose

    donor to insert iTagRFP-T at the ACTB locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    ACTB-TagRFP-T (ACTB Human)
    

    Use

    Donor template

    Tags

    TagRFP-T

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP1954

    Plasmid

    #222730

    Purpose

    pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-ACTB-C200-GFPfr1

    Plasmid

    #169798

    Purpose

    THe donor vector for the ACTB gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to ACTB gene for HR
    

    Use

    Hr donor vector for human actb gene.

    Mutation

    Partial sequence of GFP is inserted before the st…

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-KLF7-200-GFPfr1

    Plasmid

    #169799

    Purpose

    THe donor vector for the KLF7 gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to KLF7 neighbouring region for HR
    

    Use

    Hr donor vector for human actb gene.

    Mutation

    Partial sequence of GFP is inserted the sequence …

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Intron-Tagging-pX330-Cas9-mCherry

    Plasmid

    #159742

    Purpose

    Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-T2A-LexGAD-3xP3-RFP

    Plasmid

    #127558

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    LexGAD
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-ACTB-C200-T7

    Plasmid

    #229850

    Purpose

    Donor vector for the ACTB site containing T7 sequence for detection of HR products.

    Depositor

    Natsuko Chiba

    Insert

    Partial sequence of ACTB gene
    

    Use

    Hr donor vector for human actb gene.

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscAAV-Tyr

    Plasmid

    #135616

    Purpose

    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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